Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
This strain uses the standard medium without modifications.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Artificial sea water | 750.0 | ml | - | - | |
(NH4)2SO4 | 1.0 | g | 0.999 | 7.56 | |
KH2PO4 | 0.5 | g | 0.5 | 3.671 | |
Yeast extract | 2.0 | g | 1.998 | - | |
MOPS
(SIGMA) |
5.0 | g | 4.995 | 23.87 | |
Modified Wolin's mineral solution | 10.0 | ml | - | - | |
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
Wolin's vitamin solution (10x) | 1.0 | ml | - | - | |
Na2S x 9 H2O | 0.3 | g | 0.3 | 1.248 | |
Distilled water | 240.0 | ml | - | - | |
1 Dissolve ingredients except vitamins and sulfide, adjust pH to 7.0, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials (to 30% of volume) and autoclave. Add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins should be sterilized by filtration. Adjust pH of the complete medium to 7.0, if necessary. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaCl | 27.70 | g | 27.7 | 473.99 |
MgSO4 x 7 H2O | 7.00 | g | 7 | 28.4 |
MgCl2 x 6 H2O | 5.50 | g | 5.5 | 27.053 |
KCl | 0.65 | g | 0.65 | 8.719 |
NaBr | 0.10 | g | 0.1 | 0.972 |
H3BO3 | 30.00 | mg | 0.03 | 0.485 |
SrCl2 x 6 H2O | 15.00 | mg | 0.015 | 0.056 |
Citric acid | 10.00 | mg | 0.01 | 0.052 |
KI | 0.05 | mg | 5.0e-5 | 1.9e-4 |
CaCl2 x 2 H2O | 2.25 | g | 2.25 | 15.305 |
Distilled water | 1000.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
MnSO4 x H2O | 0.50 | g | 0.5 | 2.958 | |
NaCl | 1.00 | g | 1 | 17.112 | |
FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
CoSO4 x 7 H2O | 0.18 | g | 0.18 | 1.161 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
ZnSO4 x 7 H2O | 0.18 | g | 0.18 | 0.626 | |
CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
AlK(SO4)2 x 12 H2O | 0.02 | g | 0.02 | 0.042 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
Na2SeO3 x 5 H2O | 0.30 | mg | 3.0e-4 | 0.001 | |
Na2WO4 x 2 H2O | 0.40 | mg | 4.0e-4 | 0.001 | |
Distilled water | 1000.00 | ml | - | - | |
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 20 | mg | 0.02 | 0.082 |
Folic acid | 20 | mg | 0.02 | 0.045 |
Pyridoxine hydrochloride | 100 | mg | 0.1 | 0.486 |
Thiamine HCl | 50 | mg | 0.05 | 0.148 |
Riboflavin | 50 | mg | 0.05 | 0.133 |
Nicotinic acid | 50 | mg | 0.05 | 0.406 |
Calcium D-(+)-pantothenate | 50 | mg | 0.05 | 0.21 |
Vitamin B12 | 1 | mg | 1.0e-3 | 7.4e-4 |
p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 |
(DL)-alpha-Lipoic acid | 50 | mg | 0.05 | 0.242 |
Distilled water | 1000 | ml | - | - |
Last modified: | 08.04.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.0 |
Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 2 |
No modifications necessary for this strain.
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
MOPS | 4.995 | 23.87 |
MgCl2 x 6 H2O | 4.121 | 20.27 |
Yeast extract | 1.998 | - |
(NH4)2SO4 | 0.999 | 7.56 |
KH2PO4 | 0.5 | 3.671 |
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