DSMZ 917 . MODIFIED SEA WATER AGAR

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 1.50 g 1.5 -
Peptone 2.50 g 2.5 -
Hexadecane 2.00 g 2 8.832
NaCl 17.70 g 17.7 302.875
KCl 0.48 g 0.48 6.439
MgCl2 x 6 H2O 3.40 g 3.4 16.724
MgSO4 x 7 H2O 4.46 g 4.46 18.095
CaCl2 x 2 H2O 0.98 g 0.98 6.666
Agar 15.00 g 15 44.599
Distilled water 1000.00 ml - -
1 Adjust to pH 7.2. Add agar and autoclave at 121°C for 20 min. A stock solution of calcium chloride should be sterilized separately and added to the medium after autoclaving.

Metadata

Last modified: 19.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 17.7 302.875
Agar 15 44.599
MgSO4 x 7 H2O 4.46 18.095
MgCl2 x 6 H2O 3.4 16.724
Peptone 2.5 -
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Cultivation metadata from 1 strains BacDive

halophily slightly halophilic 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
oxygen facultative anaerobe 1 strain
temperature mesophilic 1 strain