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DSMZ

DSMZ 921 . METHYLOSARCINA QUISQUILLARUM AND M. FIBRATA MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 921 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution 1 (10x NMS salts) 100.0 ml - -
Solution 2 (Fe EDTA) 0.1 ml - -
Solution 3 (Sodium molybdate) 1.0 ml - -
Trace elements 1.0 ml - -
Distilled water 1000.0 ml - -
Agar , for solid medium
optional 		1.5 % 15 44.599
1 Combine Solutions 1-3 with Trace Elements and distilled water. Then dispense into growth vessels and autoclave under 50% methane in air (or strain specific other ratio) atmosphere (if not using agar).
2 Autoclave at 121 °C for 15 min.
Phosphate buffer 10.0 ml/l - -
3 Autoclave at 121 °C for 15 min.
4 Add separately autoclaved phosphate buffer (10 ml/L) to autoclaved and cooled medium. If phosphate buffer is added to warm medium, it will precipitate.
5 Liquid cultures should be grown with shaking.

Trace elements #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 25.0 mg 0.25 0.744
CuSO4 x 5 H2O 100.0 mg 1 4.005
FeSO4 x 7 H2O 50.0 mg 0.5 1.798
ZnSO4 x 7 H2O 40.0 mg 0.4 1.391
H3BO3 1.5 mg 0.015 0.243
CoCl2 x 6 H2O 5.0 mg 0.05 0.385
MnCl2 x 4 H2O 2.0 mg 0.02 0.101
NiCl2 x 6 H2O 1.0 mg 0.01 0.077
Distilled water 100.0 ml - -
1 May be stored at 4°C in the dark

Phosphate buffer #

1000
Solution occurs in 2 other media (first defined in Medium 921).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 12 H2O 71.6 g 71.6 504.371
KH2PO4 26.0 g 26 191.057
Distilled water 1000.0 ml - -
1 Dissolve in about 800 ml of water, adjust the pH to 6.8 and make up to 1 litre.

Solution 1 (10x NMS salts) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
KNO3 10 g 10 98.909
MgSO4 x 6 H2O 10 g 10 83.078
CaCl2 x 2 H2O 2 g 2 13.604
Distilled water 1000 ml - -
1 Dissolve the ingredients listed above (in that order) in about 700 ml of distilled water, and then make up to 1 litre

Solution 2 (Fe EDTA) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Fe(III)-EDTA
(Sigma, 03650) 		3.8 g 3.8 11.045
Distilled water 1000.0 ml - -

Solution 3 (Sodium molybdate) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2MoO4 x 2 H2O 0.26 g 0.26 1.075
Distilled water 1000.00 ml - -

Metadata

Last modified: 16.04.24
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 9
Strains with modifications: 6

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
KNO3 1 9.891
MgSO4 x 6 H2O 1 8.308
Na2HPO4 x 12 H2O 0.716 5.044
KH2PO4 0.26 1.911
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Cultivation metadata from 9 strains BacDive

pH 6 - 8 4 strains
pH 4 - 6 3 strains
pH 8 - 10 2 strains
phyla 2 12 strains
oxygen aerobe 6 strains
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