922
922 .
METHYLOCAPSA ACIDOPHILA MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 922 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 100 | mg | 0.1 | 0.735 | |
| MgSO4 x 7 H2O | 50 | mg | 0.05 | 0.203 | |
| CaCl2 x 2 H2O | 10 | mg | 0.01 | 0.068 | |
| Trace elements | 1 | ml | - | - | |
| Distilled water | 1000 | ml | - | - | |
| KNO3 , enhances growth | 100 | mg | 0.1 | 0.989 | |
| 1 Final pH 4.0-4.5. | |||||
| 2 The medium is fairly weakly buffered so the pH should be checked before and after autoclaving. In the early stage of growth the strain may form aggregates which break as the culture ages. 10-30% methane is added to the gas phase. Adding 100 mg/litre KNO3 enhances growth. | |||||
| 3 The strain should be grown with shaking. | |||||
Solution occurs in 2
other media (first defined in Medium 922).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| EDTA | 5.00 | g | 5 | 17.109 |
| CuCl2 x 5 H2O | 0.10 | g | 0.1 | 0.445 |
| FeSO4 x 7 H2O | 2.00 | g | 2 | 7.194 |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 |
| NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 |
| CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 |
| Na2MoO4 | 0.03 | g | 0.03 | 0.146 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 25.03.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 4.0 - 4.5 |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| KNO3 | 0.1 | 0.989 |
| KH2PO4 | 0.1 | 0.735 |
| MgSO4 x 7 H2O | 0.05 | 0.203 |
| CaCl2 x 2 H2O | 0.01 | 0.068 |
| EDTA | 0.005 | 0.017 |
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