This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na glutamate
(Sigma) |
10.000 | g | 10 | 59.133 | |
Yeast extract
(Oxoid) |
5.000 | g | 5 | - | |
Na2CO3 | 10.000 | g | 10 | 94.35 | |
K2HPO4 | 0.150 | g | 0.15 | 0.861 | |
MgSO4 x 7 H2O | 0.025 | g | 0.025 | 0.101 | |
(NH4)2SO4 | 1.000 | g | 1 | 7.568 | |
Distilled water | 1000.000 | ml | - | - | |
Agar , for solid medium | 20.000 | g | 20 | 59.465 | |
1 We recommend that liquid media be made by autoclaving the Na2CO3 separately, otherwise the high pH will cause ammonia to be lost. When making media solidified with agar (20 g/l) the agar should also not be autoclaved with the Na2CO3 otherwise the agar will be hydrolysed. When mixing the ingredients for solid media cool all components to about 55°C otherwise hydrolysis of the agar may occur. |
Last modified: | 15.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Autoclave |
Edit in Medium builder |
All strains for this medium: | 4 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Agar | 20 | 59.465 |
Na2CO3 | 10 | 94.35 |
Na glutamate | 10 | 59.133 |
Yeast extract | 5 | - |
(NH4)2SO4 | 1 | 7.568 |
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