923
923 .
ALKALIBACTERIUM OLIVAPOVLITICUS
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 923 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Na glutamate
(Sigma) |
10.000 | g | 10 | 59.133 | |
|
Yeast extract
(Oxoid) |
5.000 | g | 5 | - | |
| Na2CO3 | 10.000 | g | 10 | 94.35 | |
| K2HPO4 | 0.150 | g | 0.15 | 0.861 | |
| MgSO4 x 7 H2O | 0.025 | g | 0.025 | 0.101 | |
| (NH4)2SO4 | 1.000 | g | 1 | 7.568 | |
| Distilled water | 1000.000 | ml | - | - | |
| Agar , for solid medium | 20.000 | g | 20 | 59.465 | |
| 1 We recommend that liquid media be made by autoclaving the Na2CO3 separately, otherwise the high pH will cause ammonia to be lost. When making media solidified with agar (20 g/l) the agar should also not be autoclaved with the Na2CO3 otherwise the agar will be hydrolysed. When mixing the ingredients for solid media cool all components to about 55°C otherwise hydrolysis of the agar may occur. | |||||
Metadata
| Last modified: | 15.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 4 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 20 | 59.465 |
| Na2CO3 | 10 | 94.35 |
| Na glutamate | 10 | 59.133 |
| Yeast extract | 5 | - |
| (NH4)2SO4 | 1 | 7.568 |
| Show more | ||
