Name: ALKALIPHILIC SULPHUR RESPIRING STRAINS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 925 #

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Na₂CO₃	20	g	20	188.701
NaHCO₃	10	g	10	119.038
NaCl	5	g	5	85.558
K₂HPO₄	1	g	1	5.741
Trace element solution	2	ml/l	-	-
Distilled water	1000	ml	-	-
1 Sterilize at 110°C 20 min in a closed vessel (i.e. a serum tube or bottle). pH after sterilization will about 10.
2 After sterilization add:
MgCl₂ x 6 H₂O (200.0 g/l)	1	ml/l	0.2	0.984
3 (a white colloid will form which will rapidly dissolve after mixing)

Trace element solution #

1000

Solution occurs in 5 other media (first defined in Medium 925).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
EDTA	5	mg	0.005	0.017
FeSO₄ x 7 H₂O	2	mg	0.002	0.007
ZnSO₄ x 7 H₂O	100	mg	0.1	0.348
MnCl₂ x 4 H₂O	30	mg	0.03	0.152
CoCl₂ x 6 H₂O	200	mg	0.2	1.54
NiCl₂ x 6 H₂O	20	mg	0.02	0.154
Na₂MoO₄ x 2 H₂O	30	mg	0.03	0.124
CuCl₂ x 2 H₂O	10	mg	0.01	0.059
H₃BO₃	300	mg	0.3	4.851
Distilled water	1000	ml	-	-
1 Final pH should be 3, add HCl if needed. Sterilization - 120°C 20 min.

All strains for this medium:	13
Strains with modifications:	12

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