928 .
ROSEINATRONOBACTER MEDIUM
What is this page?
Equipment needed:
Autoclave
Final pH:
10.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2CO3 | 22.00 | g | ||
|
|
NaHCO3 | 8.00 | g | ||
|
|
NaCl | 5.00 | g | ||
|
|
KNO3 | 0.50 | g | ||
|
|
K2HPO4 | 0.50 | g | ||
|
|
Yeast extract | 1.00 | g | ||
|
|
Peptone | 1.00 | g | ||
|
|
Sodium acetate | 1.60 | g | ||
|
|
MgSO4 | 0.06 | g | ||
|
|
Trace elements solution (Pfennig & Lippert,1966) | 2.00 | ml | ||
|
|
Distilled water | 1000.00 | ml | ||
|
|
Agar
, for solid medium
optional |
20.00 | g | ||
| 1 Final pH should be about 10.0 | |||||
| 2 Note: If the medium is solidified with agar prepare the medium given above in 500 ml of distilled water and 20 g of agar in another container with 500 ml distilled water. Autoclave separately and allow to cool to about 50°C before mixing. | |||||
Trace elements solution (Pfennig & Lippert,1966) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
EDTA | 5.00 | g | ||
|
|
FeSO4 x 7 H2O | 2.20 | g | ||
|
|
ZnSO4 x 7 H2O | 0.10 | g | ||
|
|
MnCl2 x 4 H2O | 0.03 | g | ||
|
|
H3BO3 | 0.03 | g | ||
|
|
CoCl2 x 6 H2O | 0.20 | g | ||
|
|
CuCl2 x 2 H2O | 0.03 | g | ||
|
|
NiCl2 x 6 H2O | 0.03 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.03 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 pH 3.0-4.0 | |||||