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DSMZ

DSMZ 933 . HALOANAEROBIUM CONGOLENSE MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 933 #

ml
Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.00 g 1 18.695
K2HPO4 0.30 g 0.3 1.722
KH2PO4 0.30 g 0.3 2.205
MgCl2 x 6 H2O 10.00 g 10 49.188
CaCl2 x 2 H2O 0.10 g 0.1 0.68
KCl 1.00 g 1 13.414
Na-acetate 0.50 g 0.5 6.095
Cysteine 0.50 g 0.5 4.127
Trypticase 1.00 g 1 -
Yeast extract 1.00 g 1 -
NaCl 100.00 g 100 1711.16
Resazurin 1.00 mg 1.0e-3 0.004
Na2S x 9 H2O 0.40 g 0.4 1.665
NaHCO3 2.00 g 2 23.808
Glucose 3.60 g 3.6 19.982
Sodium thiosulphate 3.16 g 3.16 19.986
Modified Wolin's mineral solution II 1.00 ml - -
Distilled water 1000.00 ml - -
1 Prepare the medium and boil, cool under N2:CO2 (80:20 v/v). Dispense medium into Hungate tubes or serum bottles under N2:CO2 (80:20 v/v), autoclave. To the sterile medium add from sterile stock solutions 2% Na2S x 9 H2O (0.2 ml/10 ml), 10% NaHCO3 (0.2 ml/10 ml), 1M glucose (0.2 ml/10 ml) and 1M sodium thiosulphate (0.2 ml/10 ml). Final pH 7.0

Modified Wolin's mineral solution II #

1000
Solution occurs in 24 other media (first defined in Medium 700).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g 1.5 7.848
MgSO4 x 7 H2O 3.000 g 3 12.172
MnSO4 x H2O 0.500 g 0.5 2.958
NaCl 1.000 g 1 17.112
FeSO4 x 7 H2O 0.100 g 0.1 0.36
CoSO4 x 7 H2O 0.180 g 0.18 1.161
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnSO4 x 7 H2O 0.180 g 0.18 0.626
CuSO4 x 5 H2O 0.010 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.020 g 0.02 0.042
H3BO3 0.010 g 0.01 0.162
Na2MoO4 x 2 H2O 0.010 g 0.01 0.041
NiCl2 x 6 H2O 0.025 g 0.025 0.193
Na2SeO3 x 5 H2O 0.300 mg 3.0e-4 0.001
Distilled water 1000.000 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 08.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 10 49.188
Glucose 3.6 19.982
Sodium thiosulphate 3.16 19.986
NaHCO3 2 23.808
NH4Cl 1 18.695
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain