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DSMZ

DSMZ 939 . METHYLOTROPHIC ARTHROBACTER AND HYPHOMICROBIUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 939 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 7.9 g 7.9 55.65
KH2PO4 1.5 g 1.5 11.023
NH4Cl 0.8 g 0.8 14.956
MgSO4 x 7 H2O 0.1 g 0.1 0.406
Trace elements solution 10.0 ml - -
Distilled water 1000.0 ml - -
Methanol 10.0 ml 7.92 247.176
1 Final pH 7.2-7.5
2 After autoclaving add 10 ml methanol to 1 litre of the sterile Mineral Salts Solution.

Trace elements solution #

1000
Solution occurs in 1 other media (first defined in Medium 939).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 50.00 g 50 171.092
ZnSO4 x 7 H2O 1.00 g 1 3.478
CaCl2 x 2 H2O 7.34 g 7.34 49.927
MnCl2 x 4 H2O 2.50 g 2.5 12.632
CoCl2 x 6 H2O 0.50 g 0.5 3.851
(NH4)6Mo7O24 0.50 g 0.5 -
FeSO4 x 7 H2O 5.00 g 5 17.985
CuSO4 x 5 H2O 0.20 g 0.2 0.801
Distilled water 1000.00 ml - -
1 Dissolve the EDTA in about 400 ml of water, then add 9 g NaOH. Dissolve each of the salts individually in about 40-50 ml of water and add them to the EDTA-NaOH solution. Adjust the final pH of the solution to pH 6.0 with 1M NaOH (about 24 ml). Make up to 1 litre with distilled water, store in the dark and do not autoclave the stock solution before it is added to the medium.

Metadata

Last modified: 25.03.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.2 - 7.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 3
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Methanol 7.92 247.176
Na2HPO4 x 2 H2O 7.9 55.65
KH2PO4 1.5 11.023
NH4Cl 0.8 14.956
EDTA 0.5 1.711
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Cultivation metadata from 3 strains BacDive

phyla 2 3 strains
oxygen aerobe 1 strain
oxygen obligate aerobe 1 strain
temperature mesophilic 3 strains