942
942 .
AGAR WITH E. COLI AS FEED
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 942 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| CaCl2 x 2 H2O | 1 | g | 1 | 6.802 | |
| Agar | 15 | g | 15 | 44.599 | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 7.2, autoclave and pour plates. Grow Escherichia coli (e.g. DSM 498) in liquid culture, collect cells by centrifugation and resuspend them in enough water to receive a thick but ready-to-pipet suspension. Autoclave. Place three separate drops on one CaCl2-agar plate and streak every drop once to receive E. coli lines, about 3 cm long, across the E. coli drops. Inoculate E. coli lines/drops with myxobacteria. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 24 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| CaCl2 x 2 H2O | 1 | 6.802 |
| Distilled water | - | - |
Cultivation metadata from 24 strains
| phyla | 1 | 24 strains |
| oxygen | facultative anaerobe | 1 strain |
| temperature | mesophilic | 24 strains |