944 .
PSEUDOMONAS CHLOROTIDISMUTANS MEDIUM
What is this page?
* Medium for Pseudomonas stutzeri DSM 13592
Anaerobic. Rehydrate and grow freeze dried cells from the ampoule preferably anaerobically in medium 944.
Growth conditions: 30 °C
Equipment needed:
Anaerobic workbench
Autoclave
Filter
Final gas composition:
100 % N2
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Final pH:
8.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 900 | ml | ||
|
|
Solution B | 50 | ml | ||
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Solution C | 50 | ml | ||
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Vitamin solution | 1 | ml | ||
| 1 All solutions are prepared anaerobically under N2 and autoclaved. After cooling, the solutions are combined under N2 gas. Add 1 ml/l vitamin solution. pH should be 8.5 without further adjustment. | |||||
Solution A #
900
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
KH2PO4 | 0.41 | g |
|
|
Na2HPO4 | 0.53 | g |
|
|
NaClO3 | 1.06 | g |
|
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Na-acetate x 3 H2O | 2.72 | g |
|
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Resazurin | 0.50 | mg |
|
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Trace element solution SL-10 | 1.00 | ml |
|
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Selenite-tungstate-molybdate solution | 10.00 | ml |
|
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Distilled water | 900.00 | ml |
Solution C #
50
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NH4HCO3 | 0.44 | g |
|
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NaHCO3 | 3.73 | g |
|
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Na2S x 9 H2O | 0.50 | g |
|
|
Distilled water | 50.00 | ml |
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(25%) |
10.0 | ml | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
|
ZnCl2 | 70.0 | mg | ||
|
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
|
H3BO3 | 6.0 | mg | ||
|
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CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
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Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
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Distilled water | 990.0 | ml | ||
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Selenite-tungstate-molybdate solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaOH | 500 | mg |
|
|
Na2SeO3 x 5 H2O | 3 | mg |
|
|
Na2WO4 x 2 H2O | 3 | mg |
|
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Na2MoO4 x 2 H2O | 3 | mg |
|
|
Distilled water | 1000 | ml |
Vitamin solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Vitamin B12 | 50 | mg | ||
|
|
Pantothenic acid | 50 | mg | ||
|
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Riboflavin | 50 | mg | ||
|
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Pyridoxamine hydrochloride | 10 | mg | ||
|
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Biotin | 20 | mg | ||
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Folic acid | 20 | mg | ||
|
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Nicotinic acid | 25 | mg | ||
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Nicotine amide | 25 | mg | ||
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alpha-lipoic acid | 50 | mg | ||
|
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p-Aminobenzoic acid | 50 | mg | ||
|
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Thiamine-HCl x 2 H2O | 50 | mg | ||
|
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Distilled water | 1000 | ml | ||
| 1 Stir for some hours, filter sterilize the solution. | |||||