DSMZ 945 . MARINITOGA PIEZOPHILA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Gassing station Water bath
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.00 g 1 18.695
MgCl2 x 6 H2O 0.20 g 0.2 0.984
CaCl2 x 2 H2O 0.10 g 0.1 0.68
KCl 0.10 g 0.1 1.341
NaCl 30.00 g 30 513.347
Na-acetate 0.83 g 0.83 10.118
MES
(SIGMA)
1.95 g 1.95 9.988
Yeast extract 5.00 g 5 -
Trypticase peptone
(BD BBL)
5.00 g 5 -
K2HPO4 0.30 g 0.3 1.722
KH2PO4 0.30 g 0.3 2.205
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 -
Sulfur
(powdered)
10.00 g 10 311.818
L-Cysteine HCl x H2O 0.30 g 0.3 1.708
Na2S x 9 H2O 0.30 g 0.3 1.249
Distilled water 1000.00 ml - -
1 Dissolve ingredients except sulfur, cysteine and sulfide. Sparge medium with 100% N2 gas for at least 30 - 45 min to make it anoxic and adjust pH to 6.0 with NaOH. Distribute the medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials which contain already the appropriate amount of sulfur. For sterilization heat the cultivation vessels containing medium plus sulfur in boiling water for 2 - 3 hours on each of 3 successive days! Prior to inoculation add cysteine and sulfide and from sterile anoxic stock solutions prepared under 100% N2 gas. After inoculation pressurize tubes to 2 bar overpressure with sterile 100% N2 gas.

Metadata

Last modified: 11.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.0
Equipment needed: Gassing station, Hungate tubes, Water bath
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2
Strains with modifications: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 30 513.347
Sulfur 10 311.818
Trypticase peptone 5 -
Yeast extract 5 -
MES 1.95 9.988
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Cultivation metadata from 2 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 2 strains
oxygen anaerobe 2 strains
temperature thermophilic 2 strains