946
946 .
NAUTILIA MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 946 #
ml
Equipment needed: Hungate tubes
Filter
Gassing station
Water bath
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Synthetic seawater (2 x conc.) | 500.00 | ml | - | - | |
| NH4Cl | 0.33 | g | 0.326 | 6.102 | |
| KH2PO4 | 0.33 | g | 0.326 | 2.399 | |
| Modified Wolin's mineral solution | 10.00 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
|
Sulfur
(powdered) |
10.00 | g | 9.891 | 308.425 | |
| Na2CO3 | 1.50 | g | 1.484 | 13.999 | |
| Na-formate | 3.00 | g | 2.967 | 43.633 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| Na2S x 9 H2O | 0.60 | g | 0.593 | 2.471 | |
| Distilled water | 500.00 | ml | - | - | |
| 1 Dissolve ingredients (except sulfur, carbonate, formate, vitamins and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials that contain already the appropriate amount of sulfur. For sterilization heat cultivation vessels for 1 - 2 hours to 90 - 100°C in a water bath on each of three successive days. Prior to inoculation add carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere and formate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins are sterilized by filtration. Adjust pH of complete medium to 6.8, if necessary. | |||||
Solution occurs in 166
other media (first defined in Medium 141).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x H2O | 0.50 | g | 0.5 | 2.958 | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.18 | g | 0.18 | 1.161 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.18 | g | 0.18 | 0.626 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 x 12 H2O | 0.02 | g | 0.02 | 0.042 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
| Na2SeO3 x 5 H2O | 0.30 | mg | 3.0e-4 | 0.001 | |
| Na2WO4 x 2 H2O | 0.40 | mg | 4.0e-4 | 0.001 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. | |||||
Solution occurs in 3
other media (first defined in Medium 795).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaCl | 55.40 | g | 55.4 | 947.981 |
| SrCl2 x 6 H2O | 0.03 | g | 0.03 | 0.113 |
| MgSO4 x 7 H2O | 14.00 | g | 14 | 56.801 |
| KI | 0.10 | mg | 1.0e-4 | 3.9e-4 |
| MgCl2 x 6 H2O | 11.00 | g | 11 | 54.106 |
| Na3-citrate | 20.00 | mg | 0.02 | 0.077 |
| KCl | 1.30 | g | 1.3 | 17.438 |
| CaCl2 x 2 H2O | 1.50 | g | 1.5 | 10.203 |
| NaBr | 0.20 | g | 0.2 | 1.944 |
| H3BO3 | 0.06 | g | 0.06 | 0.97 |
| Distilled water | 1000.00 | ml | - | - |
Solution occurs in 305
other media (first defined in Medium 120).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 20 | mg | 0.02 | 0.082 |
| Folic acid | 20 | mg | 0.02 | 0.045 |
| Pyridoxine hydrochloride | 100 | mg | 0.1 | 0.486 |
| Thiamine HCl | 50 | mg | 0.05 | 0.148 |
| Riboflavin | 50 | mg | 0.05 | 0.133 |
| Nicotinic acid | 50 | mg | 0.05 | 0.406 |
| Calcium D-(+)-pantothenate | 50 | mg | 0.05 | 0.21 |
| Vitamin B12 | 1 | mg | 1.0e-3 | 7.4e-4 |
| p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 |
| (DL)-alpha-Lipoic acid | 50 | mg | 0.05 | 0.242 |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 11.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 6.8 |
| Equipment needed: | Filter, Gassing station, Hungate tubes, Water bath |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sulfur | 9.891 | 308.425 |
| MgCl2 x 6 H2O | 5.44 | 26.759 |
| Na-formate | 2.967 | 43.633 |
| Na2CO3 | 1.484 | 13.999 |
| KCl | 0.643 | 8.624 |
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