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DSMZ

DSMZ 95 . DESULFUROMONAS ACETOXIDANS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 95 #

ml
Equipment needed: Autoclave Gassing station Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 951 ml - -
Solution B 3 ml - -
Solution C 40 ml - -
Solution D 10 ml - -
1 Sparge solution A with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense solution under same gas atmosphere into anoxic Hungate-type tubes or serum vials and sterilize by autoclaving. Solutions B and D are autoclaved separately under 100% N2 gas atmosphere. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. The medium is completed before use by adding appropriate amounts of solutions B, C and D to solution A. Adjust pH of complete medium to 7.2, if necessary.

Solution A #

951
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.00 g 1.052 7.727
NH4Cl 0.30 g 0.315 5.898
MgSO4 x 7 H2O 1.00 g 1.052 4.266
MgCl2 x 6 H2O 2.00 g 2.103 10.344
NaCl 20.00 g 21.031 359.865
CaCl2 x 2 H2O 0.10 g 0.105 0.715
Na2SO4 1.77 g 1.861 13.103
Trace element solution SL-11 1.00 ml - -
Na2-DL-malate 2.66 g 2.797 15.709
Yeast extract 0.50 g 0.526 -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.3e-4 0.002
Distilled water 950.00 ml - -
1 Adjust pH to 6.0 with 2 N NaOH.

Solution B #

3
Compound Amount Unit Conc. [g/L] Conc. [mM]
Ethanol 0.3 ml - -
Distilled water 2.7 ml - -

Solution C #

40
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 1.85 g 46.25 550.549
Distilled water 40.00 ml - -

Solution D #

10
Equipment needed: Autoclave Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 0.3 g 30 124.905
Distilled water 10.0 ml - -

Trace element solution SL-11 #

1000
Solution occurs in 22 other media (first defined in Medium 722).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA x 2 H2O 5.2 g 5.2 15.467
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 1000.0 ml - -
1 Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.

Metadata

Last modified: 05.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.92 340.868
Na2-DL-malate 2.649 14.88
MgCl2 x 6 H2O 1.992 9.798
NaHCO3 1.843 21.934
Na2SO4 1.763 12.412
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
oxygen anaerobe 3 strains
temperature mesophilic 3 strains