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95. DESULFUROMONAS ACETOXIDANS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2 Solution A 942 ml - -
3 Solution B 3 ml - -
4 Solution C 40 ml - -
5 Solution D 10 ml - -
Instructions:
  1. Sparge solution A with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense solution under same gas atmosphere into anoxic Hungate-type tubes or serum vials and sterilize by autoclaving. Solutions B and D are autoclaved separately under 100% N2 gas atmosphere. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. The medium is completed before use by adding appropriate amounts of solutions B, C and D to solution A. Adjust pH of complete medium to 7.2, if necessary.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.00 g 1.062 7.801
2 0.30 g 0.318 5.954
3 1.00 g 1.062 4.307
4 2.00 g 2.123 10.443
5 20.00 g 21.231 363.287
6 0.10 g 0.106 0.722
7 1.77 g 1.879 13.228
8 Trace element solution SL-11 1.00 ml - -
9 2.66 g 2.824 15.859
10 0.50 g 0.531 -
11
(0.1% w/v)
0.50 ml 5.3e-4 0.002
12 940.00 ml 996.088 55291.2
13 , for pH adjustment
(2 N)
x - -
Instructions:
  1. Adjust pH to 6.0 with 2 N NaOH.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.3 ml 79 1714.84
2 2.7 ml 898.386 49868
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.85 g 46.25 550.552
2 40.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.3 g 30 124.905
2 10.0 ml 998.207 55408.8
Solution occurs in 22 other media (first defined in medium 722 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.2 g 5.2 13.97
2 1.5 g 1.5 7.545
3 70.0 mg 0.07 0.514
4 100.0 mg 0.1 0.505
5 6.0 mg 0.006 0.097
6 190.0 mg 0.19 0.799
7 2.0 mg 0.002 0.012
8 24.0 mg 0.024 0.101
9 36.0 mg 0.036 0.149
10 1000.0 ml 998.207 55408.8
Instructions:
  1. Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.0
Equipment needed: Autoclave, Hungate tubes

Associated strains

All strains for this medium: 3
Strains with modifications: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 991.919 55059.8
NaCl 20 342.216
Na malate 2.66 14.94
MgCl2 x 6 H2O 2 9.837
NaHCO3 1.85 22.022
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
oxygen anaerobe 3 strains
temperature mesophilic 3 strains

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