Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Casamino acids | 5.0 | g | ||
|
Yeast extract
(Oxoid) |
5.0 | g | ||
|
Tris | 12.1 | g | ||
|
KCl | 2.0 | g | ||
|
MgCl2 x 6 H2O | 20.0 | g | ||
|
CaCl2 x 2 H2O | 0.2 | g | ||
|
NaCl | 200.0 | g | ||
|
Agar | 20.0 | g | ||
|
Distilled water | 1000.0 | ml | ||
1 Adjust pH to 7.4. Add the agar after dissolving all ingredients in the water and adjustment of pH. |