959
959 .
PETROTOGA OLEARIA MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Thermosipho geolei DSM 13256
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
70 °C
Main sol. 959 #
ml
Equipment needed: Hungate tubes
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 15.00 | g | 15 | 256.674 | |
| MgCl2 x 6 H2O | 0.50 | g | 0.5 | 2.459 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| KCl | 0.20 | g | 0.2 | 2.683 | |
| PIPES | 3.40 | g | 3.4 | 11.245 | |
| K2HPO4 | 0.35 | g | 0.35 | 2.009 | |
| KH2PO4 | 0.35 | g | 0.35 | 2.572 | |
| Na-acetate x 3 H2O | 2.72 | g | 2.72 | - | |
|
Yeast extract
(OXOID) |
2.00 | g | 2 | - | |
|
Trypticase peptone
(BD BBL) |
2.00 | g | 2 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| Maltose | 3.50 | g | 3.5 | 10.225 | |
| Na2S x 9 H2O | 0.50 | g | 0.5 | 2.082 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except maltose and sulfide, adjust pH to 7.0 - 7.2 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add maltose and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. The pH of the complete medium should be 7.5. | |||||
Metadata
| Last modified: | 11.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.5 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 3 |
Thermosipho geolei DSM 13256
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 15 | 256.674 |
| Maltose | 3.5 | 10.225 |
| PIPES | 3.4 | 11.245 |
| Na-acetate x 3 H2O | 2.72 | - |
| Trypticase peptone | 2 | - |
| Show more | ||
