Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 26.00 | g | 26 | 444.901 | |
MgCl2 x 6 H2O | 0.50 | g | 0.5 | 2.459 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
NH4Cl | 1.00 | g | 1 | 18.695 | |
KCl | 0.20 | g | 0.2 | 2.683 | |
PIPES
(SIGMA) |
3.40 | g | 3.4 | 11.245 | |
K2HPO4 | 0.35 | g | 0.35 | 2.009 | |
KH2PO4 | 0.35 | g | 0.35 | 2.572 | |
Na-acetate x 3 H2O | 2.72 | g | 2.72 | - | |
Yeast extract
(OXOID) |
2.00 | g | 2 | - | |
Trypticase peptone
(BD BBL) |
2.00 | g | 2 | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
Maltose | 3.50 | g | 3.5 | 10.225 | |
Na2S x 9 H2O | 0.50 | g | 0.5 | 2.082 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients except maltose and sulfide, adjust pH to 5.8 - 6.0 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add maltose and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. The pH of the complete medium should be 6.0. |
Last modified: | 11.04.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 6.0 |
Equipment needed: | Autoclave, Gassing station, Hungate tubes |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 26 | 444.901 |
Maltose | 3.5 | 10.225 |
PIPES | 3.4 | 11.245 |
Na-acetate x 3 H2O | 2.72 | - |
Trypticase peptone | 2 | - |
Show more |