961
961 .
THERMOVIBRIO MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 961 #
ml
Equipment needed: Filter
Autoclave
Gassing station
Serum bottle
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| KNO3 | 1.0 | g | 1 | 9.891 | |
|
Yeast extract
(OXOID) |
1.0 | g | 1 | - | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
| Synthetic seawater | 1000.0 | ml | - | - | |
| 1 Dissolve resazurin in seawater, then sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into serum vials (e.g., 20 ml medium per 100 ml bottle) and autoclave. Add nitrate, yeast extract and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 6.5, if necessary. | |||||
| 2 After inoculation pressurize bottles to 2 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture. | |||||
| 3 Note: Alternatively, aged filtered seawater can be used instead of synthetic seawater. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaCl | 27.70 | g | 27.7 | 473.99 |
| MgSO4 x 7 H2O | 7.00 | g | 7 | 28.4 |
| MgCl2 x 6 H2O | 5.50 | g | 5.5 | 27.053 |
| KCl | 0.65 | g | 0.65 | 8.719 |
| KH2PO4 | 0.50 | g | 0.5 | 3.674 |
| (NH4)2SO4 | 0.25 | g | 0.25 | 1.892 |
| H3BO3 | 0.03 | g | 0.03 | 0.485 |
| NaBr | 0.10 | g | 0.1 | 0.972 |
| CaCl2 x 2 H2O | 0.75 | g | 0.75 | 5.102 |
| KI | 0.05 | mg | 5.0e-5 | 1.9e-4 |
| SrCl2 x 6 H2O | 15.00 | mg | 0.015 | 0.056 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 17.10.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.5 |
| Equipment needed: | Autoclave, Filter, Gassing station, Serum bottle |
| Edit in Medium builder | |
Gas composition
- 80 % H2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 27.7 | 473.99 |
| MgSO4 x 7 H2O | 7 | 28.4 |
| MgCl2 x 6 H2O | 5.5 | 27.053 |
| KNO3 | 1 | 9.891 |
| Yeast extract | 1 | - |
| Show more | ||
