964 .
CAMINICELLA MEDIUM
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Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final pH:
7.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Sea Salt
(SIGMA) |
30.00 | g | ||
|
|
PIPES buffer
(SIGMA) |
6.05 | g | ||
|
|
D-Glucose | 5.00 | g | ||
|
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | ||
|
|
Sulfur
(powdered) |
12.00 | g | ||
|
|
Peptone
(BD Bacto) |
1.00 | g | ||
|
|
Yeast extract
(BD Bacto) |
0.50 | g | ||
|
|
Na2S x 9 H2O | 0.50 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve ingredients (except sulfur, peptone, yeast extract, and sulfide), sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Adjust pH to 7.2 - 7.4, then dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur and autoclave at 110°C for 20 min. Add peptone, yeast extract and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The pH of the complete medium should be 7.5. | |||||