DSMZ 967 . TEREDINIBACTER TURNERAE MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 15.3 mg 0.015 0.112
Na2CO3 10.0 mg 0.01 0.094
Na2MoO4 x 2 H2O 2.5 mg 0.003 0.01
Na2-EDTA 0.5 mg 5.0e-4 0.001
Ferric ammonium citrate 3.0 mg 0.003 -
HEPES 5.2 g 5.2 21.821
Trace elements (A5 of Rippka et al., 1979) 1.0 ml - -
Sea water 750.0 ml - -
Distilled water 250.0 ml - -
Agar , for solid medium 2.0 g 2 5.947
1 Teredinibacter turnerae will grow on either Sigmacell 100 (cellulose) or Whatman filter paper "1". When grown in the absence of a nitrogen source the strains will fix nitrogen under microaerophilic conditions. Microaerophilic conditions may be achieved by adding 2 g/l agar (semi-solid) and dispensing the medium into 10 ml portions in 16 mm (outside diameter) test tubes - incubate without shaking. The strains may also be grown with gentle shaking if NH4Cl, 0.27 g/l is added to the medium.
Compound Amount Unit Conc. [g/L] Conc. [mM]
H3BO3 2.860 g 2.86 46.248
MnCl2 x 4 H2O 1.810 g 1.81 9.146
ZnSO4 x 7 H2O 0.220 g 0.22 0.765
Na2MoO4 x 2 H2O 0.390 g 0.39 1.612
CuSO4 x 5 H2O 0.079 g 0.079 0.316
Distilled water 1000.000 ml - -

Metadata

Last modified: 01.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Filter
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Associated strains

All strains for this medium: 6

Molecular composition

Compound Conc. [g/L] Conc. [mM]
HEPES 5.2 21.821
Agar 2 5.947
KH2PO4 0.015 0.112
Na2CO3 0.01 0.094
Ferric ammonium citrate 0.003 -
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Cultivation metadata from 6 strains BacDive

pH 8 - 10 3 strains
pH 10 - 12 3 strains
phyla 1 6 strains
nutrition chemoheterotroph 3 strains
oxygen aerobe 3 strains
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