DSMZ 968 . ANAEROBACILLUS ARSENICISELENATIS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

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Selected strain: Salisediminibacterium beveridgei DSM 22320

This strain uses the standard medium without modifications.

Equipment needed: Hungate tubes Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 0.10 g 0.1 0.755
MgSO4 25.00 mg 0.025 0.207
NaCl 90.00 g 89.82 1536.97
K2HPO4 0.15 g 0.15 0.859
KH2PO4 0.08 g 0.08 0.587
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
NaNO3 1.25 g 1.248 14.677
Na-DL-lactate 1.70 g 1.697 15.14
Yeast extract 0.20 g 0.2 -
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 0.002
Na2CO3 10.60 g 10.579 99.812
NaHCO3 4.20 g 4.192 49.896
L-Cysteine HCl x H2O 0.25 g 0.25 1.421
Na2S x 9 H2O 0.25 g 0.25 1.039
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except carbonate, hydrogencarbonate and reducing agents), then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add solid carbonate and bicarbonate, adjust pH to 9.8, dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before inoculation, add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 9.8, if necessary.
Solution occurs in 263 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 176 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Metadata

Last modified: 19.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.8
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 3
Salisediminibacterium beveridgei DSM 22320

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 89.82 1536.97
Na2CO3 10.579 99.812
NaHCO3 4.192 49.896
Na-DL-lactate 1.697 15.14
NaNO3 1.248 14.677
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
oxygen anaerobe 3 strains
temperature mesophilic 3 strains