968 .
ANAEROBACILLUS ARSENICISELENATIS MEDIUM
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Selected strain: Salisediminibacterium beveridgei DSM 22320
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final gas composition:
100 % N2
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Final pH:
9.8
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ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
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(NH4)2SO4 | 0.10 | g | ||
|
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MgSO4 | 25.00 | mg | ||
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NaCl | 90.00 | g | ||
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K2HPO4 | 0.15 | g | ||
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KH2PO4 | 0.08 | g | ||
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Trace element solution SL-10 | 1.00 | ml | ||
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Selenite-tungstate solution | 1.00 | ml | ||
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NaNO3 | 1.25 | g | ||
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Na-DL-lactate | 1.70 | g | ||
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Yeast extract | 0.20 | g | ||
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Sodium resazurin
(0.1% w/v) |
0.50 | ml | ||
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Na2CO3 | 10.60 | g | ||
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NaHCO3 | 4.20 | g | ||
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L-Cysteine HCl x H2O | 0.25 | g | ||
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Na2S x 9 H2O | 0.25 | g | ||
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Distilled water | 1000.00 | ml | ||
| 1 Dissolve ingredients (except carbonate, hydrogencarbonate and reducing agents), then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add solid carbonate and bicarbonate, adjust pH to 9.8, dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before inoculation, add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 9.8, if necessary. | |||||
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(25%) |
10.0 | ml | ||
|
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FeCl2 x 4 H2O | 1.5 | g | ||
|
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ZnCl2 | 70.0 | mg | ||
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MnCl2 x 4 H2O | 100.0 | mg | ||
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H3BO3 | 6.0 | mg | ||
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CoCl2 x 6 H2O | 190.0 | mg | ||
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CuCl2 x 2 H2O | 2.0 | mg | ||
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NiCl2 x 6 H2O | 24.0 | mg | ||
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Na2MoO4 x 2 H2O | 36.0 | mg | ||
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Distilled water | 990.0 | ml | ||
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Selenite-tungstate solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaOH | 0.5 | g |
|
|
Na2SeO3 x 5 H2O | 3.0 | mg |
|
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Na2WO4 x 2 H2O | 4.0 | mg |
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Distilled water | 1000.0 | ml |