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968b. MLMS-1 MEDIUM (ARSENATE)

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.10 g 0.099 0.748
2 25.00 mg 0.025 0.205
3 60.00 g 59.289 1014.48
4 0.15 g 0.148 0.851
5 0.08 g 0.079 0.581
6 Trace element solution SL-10 1.00 ml - -
7 Selenite-tungstate solution 1.00 ml - -
8
(0.1% w/v)
0.50 ml 4.9e-4 0.002
9 10.60 g 10.474 98.825
10 4.20 g 4.15 49.403
11 3.10 g 3.063 9.818
12 Vitamin solution 10.00 ml - -
13 0.82 g 0.81 3.374
14 1000.00 ml 986.371 54751.8
Instructions:
  1. Dissolve ingredients (except carbonates, arsenate, vitamins and sulfide), sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add solid carbonate and hydrogencarbonate, adjust pH to 9.8, dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or seruim vials and autoclave. Prior to inoculation, add arsenate, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 9.8, if necessary.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 9.8
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.198 55408.3
NaCl 59.289 1014.48
Na2CO3 10.474 98.825
NaHCO3 4.15 49.403
Na2HAsO4 x 7 H2O 3.063 9.818
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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