Name: MARINITHERMUS HYDROTHERMALIS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 973 #

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Yeast extract	1.00	g	1	-
Tryptone	1.00	g	1	14.069
NaCl	30.00	g	30	513.347
K₂HPO₄	0.14	g	0.14	0.804
CaCl₂ x 2 H₂O	0.14	g	0.14	0.952
NH₄Cl	0.25	g	0.25	4.674
MgSO₄ x 7 H₂O	3.40	g	3.4	13.794
MgCl₂ x 6 H₂O	4.18	g	4.18	20.56
KCl	0.33	g	0.33	4.427
NiCl₂ x 6 H₂O	0.50	mg	5.0e-4	0.004
Na₂Se₃ x 5 H₂O	0.50	mg	5.0e-4	-
Fe(NH₄)₂(SO₄)₂ x 6 H₂O	10.00	mg	0.01	-
Modified Wolin's mineral solution II	10.00	ml	-	-
Distilled water	990.00	ml	-	-
1 Final pH 7.0.

Modified Wolin's mineral solution II #

1000

Solution occurs in 24 other media (first defined in Medium 700).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Nitrilotriacetic acid	1.500	g	1.5	7.848
MgSO₄ x 7 H₂O	3.000	g	3	12.172
MnSO₄ x H₂O	0.500	g	0.5	2.958
NaCl	1.000	g	1	17.112
FeSO₄ x 7 H₂O	0.100	g	0.1	0.36
CoSO₄ x 7 H₂O	0.180	g	0.18	1.161
CaCl₂ x 2 H₂O	0.100	g	0.1	0.68
ZnSO₄ x 7 H₂O	0.180	g	0.18	0.626
CuSO₄ x 5 H₂O	0.010	g	0.01	0.04
AlK(SO₄)₂ x 12 H₂O	0.020	g	0.02	0.042
H₃BO₃	0.010	g	0.01	0.162
Na₂MoO₄ x 2 H₂O	0.010	g	0.01	0.041
NiCl₂ x 6 H₂O	0.025	g	0.025	0.193
Na₂SeO₃ x 5 H₂O	0.300	mg	3.0e-4	0.001
Distilled water	1000.000	ml	-	-
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

All strains for this medium:

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phyla	1	1 strain
temperature	thermophilic	1 strain