976
976 .
METHYLOPHAGA ALCALICA MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 976 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 1.00 | g | 1 | 7.348 | |
| KNO3 | 1.00 | g | 1 | 9.891 | |
| MgSO4 x 7 H2O | 0.22 | g | 0.22 | 0.893 | |
| NaCl | 30.00 | g | 30 | 513.347 | |
| Na2CO3 | 5.00 | g | 5 | 47.175 | |
| Trace elements solution | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| Methanol | 10.00 | ml | 7.92 | 247.176 | |
| 1 Final pH 9.5 | |||||
| 2 Prepare the medium without the Na2CO3, which can be sterilised separately by autoclaving. 10 ml/l sterile methanol is added to the cooled medium. When preparing liquid media cool the mineral salts solution and Na2CO3 to room temperature before mixing. When preparing agar add 2.0 % agar to the mineral salts solution and autoclave. Cool the Na2CO3 stock solution and agar to 50-55°C before mixing. | |||||
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Ferric citrate | 30.0 | mg | 0.03 | 0.122 |
| CaCl2 x 2 H2O | 30.0 | mg | 0.03 | 0.204 |
| MgCl2 x 4 H2O | 5.0 | mg | 0.005 | 0.053 |
| ZnSO4 x 7 H2O | 5.0 | mg | 0.005 | 0.017 |
| CuSO4 x 5 H2O | 0.5 | g | 0.5 | 2.003 |
| Distilled water | 1000.0 | ml | - | - |
| Agar , for solid medium | 2.0 | % | 20 | 59.465 |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 9.5 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 30 | 513.347 |
| Methanol | 7.92 | 247.176 |
| Na2CO3 | 5 | 47.175 |
| KNO3 | 1 | 9.891 |
| KH2PO4 | 1 | 7.348 |
| Show more | ||
