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DSMZ

DSMZ 978 . THERMOACTINOMYCES MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 978 #

ml
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Asparagine 1 g 1 7.569
Glycerol 2 g 2 21.718
K2HPO4 1 g 1 5.741
Polypeptone
(Nihon Seiyaku) 		30 g 30 -
Trace salt solution 1 ml - -
Vitamin B solution 10 ml - -
Agar 20 g 15 44.599
Distilled water 990 - -
1 Add components, except vitamin B solution, to distilled water and bring volume to 990 ml. Adjust pH to 7.2. After autoclaving, aseptically add filter-sterilized vitamin B solution.

Trace salt solution #

100
Solution occurs in 6 other media (first defined in Medium 547).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 0.1 g 1 3.597
MnCl2 x 4 H2O 0.1 g 1 5.053
ZnSO4 x 7 H2O 0.1 g 1 3.478
Distilled water 100.0 ml - -

Vitamin B solution #

200
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine HCl 10 mg 0.05 0.148
Riboflavin 10 mg 0.05 0.133
Nicotinate 10 mg 0.05 0.41
Pyridoxine hydrochloride 10 mg 0.05 0.243
Inositol 10 mg 0.05 0.278
Calcium panthothenate 10 mg 0.05 -
p-Aminobenzoate 10 mg 0.05 0.367
D-Biotin 5 mg 0.025 0.102
Distilled water 200 ml - -

Metadata

Last modified: 20.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Polypeptone 30 -
Agar 15 44.599
Glycerol 2 21.718
L-Asparagine 1 7.569
K2HPO4 1 5.741
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen aerobe 1 strain
temperature mesophilic 1 strain