978
978 .
THERMOACTINOMYCES MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 978 #
ml
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| L-Asparagine | 1 | g | 1 | 7.569 | |
| Glycerol | 2 | g | 2 | 21.718 | |
| K2HPO4 | 1 | g | 1 | 5.741 | |
|
Polypeptone
(Nihon Seiyaku) |
30 | g | 30 | - | |
| Trace salt solution | 1 | ml | - | - | |
| Vitamin B solution | 10 | ml | - | - | |
| Agar | 20 | g | 15 | 44.599 | |
| Distilled water | 990 | - | - | ||
| 1 Add components, except vitamin B solution, to distilled water and bring volume to 990 ml. Adjust pH to 7.2. After autoclaving, aseptically add filter-sterilized vitamin B solution. | |||||
Solution occurs in 6
other media (first defined in Medium 547).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| FeSO4 x 7 H2O | 0.1 | g | 1 | 3.597 |
| MnCl2 x 4 H2O | 0.1 | g | 1 | 5.053 |
| ZnSO4 x 7 H2O | 0.1 | g | 1 | 3.478 |
| Distilled water | 100.0 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Thiamine HCl | 10 | mg | 0.05 | 0.148 |
| Riboflavin | 10 | mg | 0.05 | 0.133 |
| Nicotinate | 10 | mg | 0.05 | 0.41 |
| Pyridoxine hydrochloride | 10 | mg | 0.05 | 0.243 |
| Inositol | 10 | mg | 0.05 | 0.278 |
| Calcium panthothenate | 10 | mg | 0.05 | - |
| p-Aminobenzoate | 10 | mg | 0.05 | 0.367 |
| D-Biotin | 5 | mg | 0.025 | 0.102 |
| Distilled water | 200 | ml | - | - |
Metadata
| Last modified: | 20.09.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Polypeptone | 30 | - |
| Agar | 15 | 44.599 |
| Glycerol | 2 | 21.718 |
| L-Asparagine | 1 | 7.569 |
| K2HPO4 | 1 | 5.741 |
| Show more | ||
