This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
K2HPO4 | 0.3 | g | 0.3 | 1.722 | |
KH2PO4 | 0.3 | g | 0.3 | 2.205 | |
MgCl2 x 6 H2O | 0.2 | g | 0.2 | 0.984 | |
CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.68 | |
KCl | 0.1 | g | 0.1 | 1.341 | |
NaCl | 1.0 | g | 1 | 17.112 | |
NH4Cl | 1.0 | g | 1 | 18.695 | |
Yeast extract | 1.0 | g | 1 | - | |
Wolin's vitamin solution | 5.0 | ml | - | - | |
Modified Wolin's mineral solution II | 10.0 | ml | - | - | |
Distilled water | 1000.0 | ml | - | - | |
1 When grown aerobically prepare the medium without the vitamins, adjust the pH to 7.0 with 10M KOH, and sterilise for 45 min at 110°C. On cooling add the vitamins from a filter sterilised stock solution. Both Geococcus nitratireducens (DSM 15495) and Petrobacter succinimandens (DSM 15512) are supplied from aerobically grown cultures. | |||||
2 Anaerobic growth may be obtained by gassing the medium with N2:CO2 (80:20), to which 10mM KNO3 has been added, and dispensing the medium under N2:CO2 (80:20). Sterilise by autoclaving at 110°C for 45 min , and to the cold medium add the filter sterilised vitamins. A reducing agent is not required. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium D-(+)-pantothenate | 5.0 | mg | 0.005 | 0.021 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
(DL)-alpha-Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.500 | g | 1.5 | 7.848 | |
MgSO4 x 7 H2O | 3.000 | g | 3 | 12.172 | |
MnSO4 x H2O | 0.500 | g | 0.5 | 2.958 | |
NaCl | 1.000 | g | 1 | 17.112 | |
FeSO4 x 7 H2O | 0.100 | g | 0.1 | 0.36 | |
CoSO4 x 7 H2O | 0.180 | g | 0.18 | 1.161 | |
CaCl2 x 2 H2O | 0.100 | g | 0.1 | 0.68 | |
ZnSO4 x 7 H2O | 0.180 | g | 0.18 | 0.626 | |
CuSO4 x 5 H2O | 0.010 | g | 0.01 | 0.04 | |
AlK(SO4)2 x 12 H2O | 0.020 | g | 0.02 | 0.042 | |
H3BO3 | 0.010 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.010 | g | 0.01 | 0.041 | |
NiCl2 x 6 H2O | 0.025 | g | 0.025 | 0.193 | |
Na2SeO3 x 5 H2O | 0.300 | mg | 3.0e-4 | 0.001 | |
Distilled water | 1000.000 | ml | - | - | |
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. |
Last modified: | 25.03.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.0 |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NH4Cl | 1 | 18.695 |
Yeast extract | 1 | - |
KH2PO4 | 0.3 | 2.205 |
K2HPO4 | 0.3 | 1.722 |
MgCl2 x 6 H2O | 0.2 | 0.984 |
Show more |