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98. RHIZOBIUM MEDIUM

Culture medium recipe

Vol.
×

M 98 #

ml
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1 g 0.833 -
2 10 g 8.333 45.744
3 15 g 12.5 -
4 200 ml - -
5 800 ml 665.471 36939.2
Instructions:
  1. Adjust pH to 7.0.
Equipment needed: Autoclave Centrifuge
# Compound Amount Unit Conc. [g/L] Conc. [mM]
6
(Air-dried)
80.0 g 400 -
7 0.2 g 1 9.435
8 200.0 ml 998.207 55408.8
Instructions:
  1. Autoclave soil suspension for one hour at 121°C. To obtain a clear supernatant allow to settle and centrifuge. Adjust pH to 7.2.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Centrifuge

Associated strains

All strains for this medium: 149
Strains with modifications: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 665.471 36939.2
Agar 12.5 -
Mannitol 8.333 45.744
Yeast extract 0.833 -
Soil extract - -

Cultivation metadata from 149 strains BacDive

halophily not 11 strains
halophily slightly 7 strains
halophily moderate 2 strains
pH 4 - 6 18 strains
pH 6 - 8 16 strains
pH 8 - 10 15 strains
pH 10 - 14 8 strains
pH 2 - 4 1 strain
phyla 1 140 strains
oxygen aerobe 18 strains
oxygen obligate aerobe 1 strain
oxygen facultative anaerobe 1 strain
temperature mesophilic 138 strains
temperature psychrophilic 4 strains
temperature thermophilic 1 strain

Similar media

1545. MODIFIED YMA MEDIUM (HAMBI) 89 %
J840. YPM MEDIUM 87 %
360. YPM MEDIUM 87 %
1334. MODIFIED YMA MEDIUM 87 %
1031. YMA-MEDIUM 86 %

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