DSMZ 98 . RHIZOBIUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Selected strain: Rhizobium smilacinae DSM 100675

This strain uses the standard medium without modifications.

Equipment needed: Centrifuge Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 1 g 1 -
Mannitol 10 g 10 54.894
Agar 15 g 15 44.599
Soil extract 200 ml - -
Distilled water 800 ml - -
1 Adjust pH to 7.0.
Compound Amount Unit Conc. [g/L] Conc. [mM]
air-dried garden soil 80.0 g 400 -
Na2CO3 0.2 g 1 9.435
Distilled water 200.0 ml - -
1 Autoclave soil suspension for one hour at 121°C. To obtain a clear supernatant allow to settle and centrifuge. Adjust pH to 7.2.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Centrifuge
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Associated strains

All strains for this medium: 149
Strains with modifications: 3
Rhizobium smilacinae DSM 100675

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
air-dried garden soil 80 -
Agar 15 44.599
Mannitol 10 54.894
Yeast extract 1 -
Na2CO3 0.2 1.887
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Cultivation metadata from 149 strains BacDive

pH 6 - 8 16 strains
pH 4 - 6 12 strains
pH 8 - 10 10 strains
pH 10 - 12 5 strains
phyla 1 157 strains
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