990
990 .
YPS MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Thermococcus marinus DSM 15227
This strain uses the standard medium without modifications.
Growth conditions: 88 °C
Main sol. 990 #
ml
Equipment needed: Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Sea Salt
(Sigma) |
35.00 | g | 35 | - | |
| PIPES | 3.46 | g | 3.46 | 11.443 | |
| Yeast extract | 1.00 | g | 1 | - | |
| Peptone | 4.00 | g | 4 | - | |
| Elemental sulphur | 5.00 | g | 5 | - | |
| NH4Cl | 0.50 | g | 0.5 | 9.348 | |
| KH2PO4 | 0.35 | g | 0.35 | 2.572 | |
| CaCl2 | 0.20 | g | 0.2 | 1.802 | |
| FeCl3 | 6.70 | mg | 0.007 | 0.041 | |
| Na2WO4 | 2.90 | mg | 0.003 | 0.01 | |
| Resazurin | 0.10 | mg | 1.0e-4 | 4.4e-4 | |
| Na2S x 9 H2O | 0.25 | g | 0.25 | 1.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Prepare the medium without the yeast extract, peptone and sodium sulphide. Boil the medium and cool under nitrogen. Adjust the pH to 6.8. Dispense into hungate tubes or serum bottles under a nitrogen atmosphere. Sterilise the medium at 100°C for 3 hours on 3 consecutive days. Add the peptone and yeast extract from sterile (10% w/v) stock solutions. The medium is reduced by adding a sterile, neutralised 5% w/v sodium sulphide solution to an end concentration of 0.025%. Final pH should be 6.8 | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | 6.8 |
| Equipment needed: | Hungate tubes |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 3 |
Thermococcus marinus DSM 15227
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sea Salt | 35 | - |
| Elemental sulphur | 5 | - |
| Peptone | 4 | - |
| PIPES | 3.46 | 11.443 |
| Yeast extract | 1 | - |
| Show more | ||
