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DSMZ

DSMZ 992 . BACILLUS FILIFORMIS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 992 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 10 g 10 -
Sodium citrate 3 g 3 11.625
KCl 2 g 2 26.828
MgSO4 x 7 H2O 1 g 1 4.057
Na2CO3 3 g 3 28.305
NaCl 100 g 100 1711.16
MnCl2 solution 0.36 g/L 1 ml - -
FeSO4 solution 5%(w/v) 1 ml - -
Distilled water 1000 ml - -
1 Sodium carbonate and sodium chloride should separately be autoclaved. pH 9.0.

MnCl2 solution 0.36 g/L #

1000
Solution occurs in 1 other media (first defined in Medium 992).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MnCl2 x 4 H2O 0.36 g 0.36 1.819
Distilled water 1000.00 ml - -

FeSO4 solution 5%(w/v) #

1000
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 50 g 50 179.846
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.0
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 2
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 100 1711.16
Yeast extract 10 -
Na2CO3 3 28.305
Sodium citrate 3 11.625
KCl 2 26.828
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Cultivation metadata from 2 strains BacDive

pH 8 - 10 2 strains
pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 2 strains
oxygen aerobe 1 strain
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