995
995 .
NPB MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 995 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Tryptone peptone
(Difco) |
10.0 | g | 10 | - | |
|
Yeast extract
(Difco) |
2.0 | g | 2 | - | |
| MgSO4 x 7 H2O | 1.0 | g | 1 | 4.057 | |
| K2HPO4 | 1.0 | g | 1 | 5.741 | |
| KH2PO4 | 0.5 | g | 0.5 | 3.674 | |
| D-Glucose | 5.0 | g | 5 | 27.753 | |
| Distilled water | 1000.0 | ml | - | - | |
| Agar , for solid medium | 15.0 | g | 15 | 44.599 | |
| 1 For solid medium 15 g of agar is mixed, and autoclaved. pH 7.0. | |||||
| 2 Inoculate the cells to 5 ml of NPB medium, and incubate at 30°C with shaking (160 strokes per min.). Cell turbidity at 660 nm after 4 days of cultivation will be 0.4-0.5. On NPB agar plate, colony formation is observed after 3-4 days of incubation at 30°C. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Tryptone peptone | 10 | - |
| D-Glucose | 5 | 27.753 |
| Yeast extract | 2 | - |
| K2HPO4 | 1 | 5.741 |
| Show more | ||
