Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
NaNO3 | 1.0 | g | ||
|
NaHCO3 | 1.0 | g | ||
|
Na2WO4 x 2 H2O | 0.1 | mg | ||
|
Na2S | 0.5 | g | ||
|
Wolin's vitamin solution | 10.0 | ml | ||
|
Synthetic seawater | 1000.0 | ml | ||
|
NaNO3 | 100.0 | g/l | ||
|
NaHCO3 | 100.0 | g/l | ||
|
Na2S | 100.0 | g/l | ||
1 To prepare the medium, all compounds of DMJ seawater were dissolved in 1 liter of distilled deionized water, and the pH was adjusted to around 7.0 with NaOH at room temperature prior to autoclaving. After autoclaving, filter-sterilized NaNO3 solution (100 g/l), NaHCO3 solution (100 g/l), Na2S solution (100 g/l); pH 7.5) and trace vitamin solution were added. Then the tubes were tightly sealed with butyl rubber stoppers under a gas phase of 80% H2 + 20% CO2 (300 kPa). |
Compound | Amount | Unit | |
---|---|---|---|
|
NaCl | 30.00 | g |
|
K2HPO4 | 0.14 | g |
|
CaCl2 x 2 H2O | 0.14 | g |
|
NH4Cl | 0.25 | g |
|
MgSO4 x 7 H2O | 3.40 | g |
|
MgCl2 x 6 H2O | 4.18 | g |
|
KCl | 0.33 | g |
|
NiCl2 x 6 H2O | 0.50 | mg |
|
Na2SeO3 x 5 H2O | 0.50 | mg |
|
Fe(NH4)2(SO4)2 x 6 H2O | 0.01 | g |
|
Trace mineral solution | 10.00 | ml |
Compound | Amount | Unit | |
---|---|---|---|
|
C6H9NO6 | 1.500 | g |
|
MgSO4 x 7 H2O | 3.000 | g |
|
MnSO4 x 2 H2O | 0.500 | g |
|
NaCl | 1.000 | g |
|
FeSO4 x 7 H2O | 0.100 | g |
|
CoSO4 x 7 H2O | 0.180 | g |
|
CaCl2 x 2 H2O | 0.100 | g |
|
ZnSO4 x 7 H2O | 0.180 | g |
|
CuSO4 x 5 H2O | 0.010 | g |
|
AlK(SO4)2 x 12 H2O | 0.020 | g |
|
H3BO3 | 0.010 | g |
|
Na2MoO4 x 2 H2O | 0.010 | g |
|
NiCl2 x 6 H2O | 0.025 | g |
|
Na2SeO3 x 5 H2O | 0.300 | mg |
|
Distilled water | 1000.000 | ml |
Compound | Amount | Unit | |
---|---|---|---|
|
Biotin | 2.0 | mg |
|
Folic acid | 2.0 | mg |
|
Pyridoxine hydrochloride | 10.0 | mg |
|
Thiamine HCl | 5.0 | mg |
|
Riboflavin | 5.0 | mg |
|
Nicotinic acid | 5.0 | mg |
|
Calcium D-(+)-pantothenate | 5.0 | mg |
|
Vitamin B12 | 0.1 | mg |
|
p-Aminobenzoic acid | 5.0 | mg |
|
(DL)-alpha-Lipoic acid | 5.0 | mg |
|
Distilled water | 1000.0 | ml |