997 .
HNW MEDIUM
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Equipment needed:
Filter
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaNO3 | 1.0 | g | ||
|
|
NaHCO3 | 1.0 | g | ||
|
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Na2WO4 x 2 H2O | 0.1 | mg | ||
|
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Na2S | 0.5 | g | ||
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Wolin's vitamin solution | 10.0 | ml | ||
|
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Synthetic seawater | 1000.0 | ml | ||
|
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NaNO3 | 100.0 | g/l | ||
|
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NaHCO3 | 100.0 | g/l | ||
|
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Na2S | 100.0 | g/l | ||
| 1 To prepare the medium, all compounds of DMJ seawater were dissolved in 1 liter of distilled deionized water, and the pH was adjusted to around 7.0 with NaOH at room temperature prior to autoclaving. After autoclaving, filter-sterilized NaNO3 solution (100 g/l), NaHCO3 solution (100 g/l), Na2S solution (100 g/l); pH 7.5) and trace vitamin solution were added. Then the tubes were tightly sealed with butyl rubber stoppers under a gas phase of 80% H2 + 20% CO2 (300 kPa). | |||||
Synthetic seawater #
10
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaCl | 30.00 | g |
|
|
K2HPO4 | 0.14 | g |
|
|
CaCl2 x 2 H2O | 0.14 | g |
|
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NH4Cl | 0.25 | g |
|
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MgSO4 x 7 H2O | 3.40 | g |
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MgCl2 x 6 H2O | 4.18 | g |
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KCl | 0.33 | g |
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NiCl2 x 6 H2O | 0.50 | mg |
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Na2SeO3 x 5 H2O | 0.50 | mg |
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Fe(NH4)2(SO4)2 x 6 H2O | 0.01 | g |
|
|
Trace mineral solution | 10.00 | ml |
Trace mineral solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
C6H9NO6 | 1.500 | g |
|
|
MgSO4 x 7 H2O | 3.000 | g |
|
|
MnSO4 x 2 H2O | 0.500 | g |
|
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NaCl | 1.000 | g |
|
|
FeSO4 x 7 H2O | 0.100 | g |
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CoSO4 x 7 H2O | 0.180 | g |
|
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CaCl2 x 2 H2O | 0.100 | g |
|
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ZnSO4 x 7 H2O | 0.180 | g |
|
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CuSO4 x 5 H2O | 0.010 | g |
|
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AlK(SO4)2 x 12 H2O | 0.020 | g |
|
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H3BO3 | 0.010 | g |
|
|
Na2MoO4 x 2 H2O | 0.010 | g |
|
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NiCl2 x 6 H2O | 0.025 | g |
|
|
Na2SeO3 x 5 H2O | 0.300 | mg |
|
|
Distilled water | 1000.000 | ml |
Wolin's vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
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Pyridoxine hydrochloride | 10.0 | mg |
|
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Thiamine HCl | 5.0 | mg |
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Riboflavin | 5.0 | mg |
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Nicotinic acid | 5.0 | mg |
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Calcium D-(+)-pantothenate | 5.0 | mg |
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Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
(DL)-alpha-Lipoic acid | 5.0 | mg |
|
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Distilled water | 1000.0 | ml |