9a
9a .
VY/2, REDUCED MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 9a #
ml
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Baker's yeast | 2.50 | g | 2.5 | - | |
| CaCl2 x 2 H2O | 1.36 | g | 1.36 | 9.251 | |
| Vitamin B12 | 0.50 | mg | 5.0e-4 | 3.7e-4 | |
|
Agar
(Difco) |
8.00 | g | 8 | 23.786 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Sterilize vitamin B12 separately by filtration. Prepare and store yeast cells as autoclaved stock suspension (5 g baker's yeast/100 ml distilled water, adjust pH to 6.5 and autoclave). | |||||
| 2 Adjust pH of medium to 7.2 with KOH before as well as after autoclaving and cooling to 50°C (use pH-indicator paper). | |||||
| 3 For suspension of freeze-dried cells from ampoules add about 0.5 - 1.0 ml medium MD1 (per liter: casiton, 3.0 g; CaCl2 x 2 H2O, 0.7 g; MgSO4 x 7 H2O, 2.0 g) to the vial with freeze dried material. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 4 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 8 | 23.786 |
| Baker's yeast | 2.5 | - |
| CaCl2 x 2 H2O | 1.36 | 9.251 |
| Vitamin B12 | 5.0e-4 | 3.7e-4 |
| Distilled water | - | - |
