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9a. VY/2, DILUTED MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.50 g 2.5 -
2 1.36 g 1.36 9.251
3 0.50 mg 5.0e-4 3.2e-4
4
(Difco)
8.00 g 8 -
5 1000.00 ml 998.207 55408.8
6 , required for pH adjustment x - -
8 Main sol. 1118 , for suspension of freeze-dried cells from ampoules 1.00 ml - -
Instructions:
  1. Sterilize vitamin B12 separately by filtration. Prepare and store yeast cells as autoclaved stock suspension ( 5 g baker's yeast/ 100 ml distilled water, adjust pH to 6.5 and autoclave). Adjust pH of medium to 7.2 with KOH before, and after autoclaving and cooling to 50°C (use pH-indicator paper). For suspension of freeze-dried cells from ampoules add about 0.5 - 1.0 ml medium 1118 (per liter: casiton 3.0 g; calciumchloride dihydrate 0.7 g; magnesiumsulphate heptahydrate 2.0 g) to the vial with freeze dried material.
Solution occurs in 2 other media (first defined in medium 1118 )
Equipment needed: Filter Autoclave Ice bath
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3.0 g 3 -
2 0.7 g 0.7 4.761
3 2.0 g 2 8.114
5 Trace element solution SL-4 1.0 ml - -
6 1000.0 ml 998.207 55408.8
Instructions:
  1. After autoclaving and cooling, add 0.5 mg /l vitamin B12 from a filter sterilized stock solution. pH should be 7.0 -7.2.
Solution occurs in 2 other media (first defined in medium 1118 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.500 g 0.5 1.723
2 0.200 g 0.2 0.719
3 0.010 g 0.01 0.035
4 0.003 g 0.003 0.015
5 0.030 g 0.03 0.485
6 0.020 g 0.02 0.084
7 0.001 g 1.0e-3 0.006
8 0.002 g 0.002 0.008
9 0.003 g 0.003 0.012
10 1000.000 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Autoclave, Filter, Ice bath

Associated strains

All strains for this medium: 4
9 VY/2 AGAR
a VY/2, DILUTED MEDIUM

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 999.206 55464.3
Agar 8 -
Baker's yeast 2.5 -
CaCl2 x 2 H2O 1.361 9.256
Casitone 0.003 -
MgSO4 x 7 H2O 0.002 0.008
Vitamin B12 5.0e-4 3.2e-4
EDTA 5.0e-7 1.7e-6
FeSO4 x 7 H2O 2.0e-7 7.2e-7
H3BO3 3.0e-8 4.9e-7
CoCl2 x 6 H2O 2.0e-8 8.4e-8
ZnSO4 x 7 H2O 1.0e-8 3.5e-8
MnCl2 x 4 H2O 3.0e-9 1.5e-8
Na2MoO4 x 2 H2O 3.0e-9 1.2e-8
NiCl2 x 6 H2O 2.0e-9 8.4e-9
CuCl2 x 2 H2O 1.0e-9 5.9e-9

Cultivation metadata from 4 strains BacDive

phyla 1 4 strains
temperature mesophilic 4 strains

Similar media

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474. MEDIUM WITH POLYHYDROXYBUTYRIC ACID AS CARBON SOURCE 76 %
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1324. MODIFIED METHYLOBACTERIUM MEDIUM 69 %
1102. MEDIUM FOR ENHYDROMYXA SALSUGINOSA 69 %

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