C105
105 .
Walne's
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Mail sol. C105 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nutrient solution | 1.0 | ml | - | - | |
| Vitamin solution | 0.1 | ml | - | - | |
|
Sea water
(sterilized) |
1000.0 | ml | - | - | |
| 1 Dispense nutrient and vitamin solutions separately into 10 ml and 1 ml respectively and autoclave at 15 psi for 15 minutes. Add an aliquot of each aseptically to 10 litres of sterilised seawater. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| FeCl3 x 6 H2O | 1.30 | g | 1.3 | 4.81 | |
| MnCl2 x 4 H2O | 0.36 | g | 0.36 | 1.819 | |
| H3BO3 | 33.60 | g | 33.6 | 543.338 | |
| Na2-EDTA | 45.00 | g | 45 | 133.847 | |
| NaH2PO4 x 2 H2O | 20.00 | g | 20 | 166.699 | |
| NaNO3 | 100.00 | g | 100 | 1176.54 | |
| Trace metal solution (TMS) | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Make up to 1 litre with distilled water. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Cyanocobalamine | 10 | mg | 0.1 | 0.074 | |
| Thiamine HCl | 10 | mg | 0.1 | 0.296 | |
| Biotin | 200 | μg | - | - | |
| Distilled water | 100 | ml | - | - | |
| 1 Make up to 100 ml with distilled water. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| ZnCl2 | 2.1 | g | 21 | 154.072 | |
| CoCl2 x 6 H2O | 2.0 | g | 20 | 154.037 | |
| (NH4)6Mo7O24 x 4 H2O | 0.9 | g | 9 | 7.282 | |
| CuSO4 x 5 H2O | 2.0 | g | 20 | 80.101 | |
| Distilled water | 100.0 | ml | - | - | |
| 1 Make up to 100 ml with distilled water. This solution is normally cloudy. Acidify with a few drops of conc. HCl to give a clear solution. | |||||
Metadata
| Last modified: | 07.10.22 |
| Source: | CCAP |
| Synonyms: | Walne's Medium |
| Medium type: | Complex medium |
| Final pH: | n.d. |
|
Description:
Reference: Walne PR (1970) Studies on the food value of nineteen genera of algae to juvenile bivalves of the genera Ostrea, Crassostrea, Mercenaria, and Mytilis. Fish. Invest. 26, 162. |
|
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