C108
108 .
ZM/10
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Mail sol. C108 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Bacto peptone | 0.5 | g | 0.5 | - | |
| Yeast extract | 0.1 | g | 0.1 | - | |
|
Agar
(Bacto) |
15.0 | g | 15 | - | |
| Marine Supplement Stock | 10.0 | ml | - | - | |
|
Sea water
(filtered) |
750.0 | ml | - | - | |
| Deionized water | 250.0 | ml | - | - | |
| 1 Make up to 1 litre with 75% filtered seawater: 25% deionized water. Autoclave at 15 psi for 15 minutes. Cool to approx. 55 °C and add sterile marine supplement. | |||||
| 1 First prepare primary stocks for marine supplement stock: | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Trace elements (x10 concentration) | 1 | ml | - | - | |
| Vitamin mix | 1 | ml | - | - | |
|
Na2SeO3
(0.002 g/l stock solution) |
1 | ml | - | - | |
| 2 Autoclave at 15 psi for 15 minutes. Once at room temperature add Primary stock 2 (Vitamin mix). | |||||
Solution occurs in 4
other media (first defined in Medium C34).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Na2-EDTA | 8.320 | g | 41.6 | 123.734 |
| FeCl3 x 6 H2O | 6.300 | g | 31.5 | 116.539 |
| CuSO4 x 5 H2O | 0.020 | g | 0.1 | 0.401 |
| ZnSO4 x 7 H2O | 0.044 | g | 0.22 | 0.765 |
| CoCl2 x 6 H2O | 0.020 | g | 0.1 | 0.77 |
| MnCl2 x 4 H2O | 0.360 | g | 1.8 | 9.095 |
| Na2MoO4 x 2 H2O | 0.012 | g | 0.06 | 0.248 |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Cyanocobalamin | 0.001 | g | 1.0e-3 | 7.4e-4 | |
| Biotin | 0.020 | g | 0.02 | 0.082 | |
| Thiamine HCl | 0.050 | g | 0.05 | 0.148 | |
| Calcium pantothenate | 0.050 | g | 0.05 | 0.105 | |
| Folic acid | 0.020 | g | 0.02 | 0.045 | |
| Riboflavin | 0.050 | g | 0.05 | 0.133 | |
| Nicotinamide | 0.050 | g | 0.05 | 0.409 | |
| 1 Dissolve vitamins one at a time; adjust the pH to 7 with 1 M NaOH or 1 M HCl if necessary, to dissolve the biotin and folic acid. Filter sterilise. Dispense excess into 1 ml aliquots and freeze. | |||||
Metadata
| Last modified: | 07.10.22 |
| Source: | CCAP |
| Synonyms: | Zobell Marine Agar 10 Medium |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Reference: | doi.org/10.1016/S0168-6496(03)00298-8 |
| Edit in Medium builder | |