CCAP 13 . BB

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaNO3
(10 g/400 ml stock solution)
10 ml - -
MgSO4 x 7 H2O
(3 g/400 ml stock solution)
10 ml - -
NaCl
(1 g/400 ml stock solution)
10 ml - -
K2HPO4
(3 g/400 ml stock solution)
10 ml - -
KH2PO4
(7 g/400 ml stock solution)
10 ml 23.4 171.951
CaCl2 x 2 H2O
(1 g/400 ml stock solution)
10 ml - -
Trace elements solution
(autoclave to dissolve)
1 ml - -
H3BO3
(11.42)
1 ml - -
Stock solution 1 1 ml - -
Stock solution 2 1 ml - -
Distilled water 1000 ml - -
1 Make up to 1 litre with glass distilled or deionised water.
Solution occurs in 1 other media (first defined in Medium C13).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 8.82 g 8.82 30.672
MnCl2 x 4 H2O 1.44 g 1.44 7.276
MoO3 0.71 g 0.71 4.932
CuSO4 x 5 H2O 1.57 g 1.57 6.288
Co(NO3)2 x 6 H2O 0.49 g 0.49 1.684
Solution occurs in 1 other media (first defined in Medium C13).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 50 g 50 171.092
KOH 31 g 31 552.526
Solution occurs in 1 other media (first defined in Medium C13).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 4.98 g 4.98 17.913
H2SO4
(concentrated)
1.00 ml - -

Metadata

Last modified: 07.10.22
Source: CCAP
Synonyms: Bold's Basal Medium
Medium type: Defined medium
Final pH: n.d.
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Associated strains

All strains for this medium: 21
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
KH2PO4 23.4 171.951
EDTA 0.05 0.171
KOH 0.031 0.553
ZnSO4 x 7 H2O 0.009 0.031
FeSO4 x 7 H2O 0.005 0.018
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