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CCAP 16 . BB:MErds

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Mail sol. C16 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Bold's Basal Medium (BB) 800 ml - -
MErds (Modified Føyns Erdschreiber Medium) 200 ml - -
1 8:2 mixture or 1:1 mixture
2 See separate recipes. Mix then autoclave at 15 psi for 15 minutes.

Bold's Basal Medium (BB) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaNO3
(10 g/400 ml stock solution) 		10 ml - -
MgSO4 x 7 H2O
(3 g/400 ml stock solution) 		10 ml - -
NaCl
(1 g/400 ml stock solution) 		10 ml - -
K2HPO4
(3 g/400 ml stock solution) 		10 ml - -
KH2PO4
(7 g/400 ml stock solution) 		10 ml 23.4 171.951
CaCl2 x 2 H2O
(1 g/400 ml stock solution) 		10 ml - -
Trace elements solution 1 ml - -
H3BO3
(11.42 g/l stock solution) 		1 ml - -
Stock solution 1 1 ml - -
Stock solution 2 1 ml - -
Distilled water 1000 ml - -
1 Make up to 1 litre with glass distilled or deionised water.

Stock solution 1 #

1000
Solution occurs in 1 other media (first defined in Medium C13).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 50 g 50 171.092
KOH 31 g 31 552.526

Stock solution 2 #

1000
Solution occurs in 1 other media (first defined in Medium C13).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 4.98 g 4.98 17.913
H2SO4
(concentrated) 		1.00 ml - -

Trace elements solution #

1000
Solution occurs in 1 other media (first defined in Medium C13).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 8.82 g 8.82 30.672
MnCl2 x 4 H2O 1.44 g 1.44 7.276
MoO3 0.71 g 0.71 4.932
CuSO4 x 5 H2O 1.57 g 1.57 6.288
Co(NO3)2 x 6 H2O 0.49 g 0.49 1.684

MErds (Modified Føyns Erdschreiber Medium) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
SES (Soil Extract with Added Salts) 100 ml - -
NaNO3
(20 g/100 ml stock solution) 		1 ml - -
Na2HPO4
(1.2 g/100 ml stock solution) 		1 ml - -
Sea water
(filtered) 		1000 ml - -
1 Mix the above constituents and autoclave at 15 psi for 15 minutes. It may be necessary to filter final medium to avoid problems with precipitate.

SES (Soil Extract with Added Salts) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4
(1.0 g/l stock solution) 		20 ml - -
MgSO4 x 7 H2O
(1.0 g/l stock solution) 		20 ml - -
KNO3
(10.0 g/l stock solution) 		20 ml - -
SE1 (Soil Extract 1) 100 ml - -
Deionized water 840 ml - -
1 Make up to 1 litre with deionized water and autoclave at 15 psi for 15 minutes.
2 At the CCAP, SE1 is used for marine algae, SE2 for freshwater and terrestrial protozoa

SE1 (Soil Extract 1) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Soil
(air-dried) 		trace - -
Distilled water trace - -
1 Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.
2 A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and handpicked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry sieve through a finer mesh (2-4 mm) and store in an airtight container away from light and heat.
3 Soil is prepared as above. Air­-dried soil and twice its volume of supernatant distilled water are autoclaved together at 15 psi for 2 hours and left to cool. The supernatant is then decanted and filtered through Whatman No 1 filter paper, then distributed to containers in volumes suitable for making up batches of media. The aliquots and their containers are autoclaved for an appropriate length of time (e.g. 1 litre or less for 15 minutes) and are then kept in a cool place (e.g. a refrigerator) until required.

SE2 (Soil Extract 2) #

660
Compound Amount Unit Conc. [g/L] Conc. [mM]
Soil
(air­dried, sieved) 		105 g 159.091 -
Deionized water 660 ml - -
1 Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.
2 A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and hand picked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry it may be sieved through a finer mesh (2­4 mm) or stored as it is prior to use.
3 Soil is prepared as above. 105 g of air­dried sieved soil and 660 ml of deionized water are placed in a 1 litre bottle and autoclaved once at 15 psi for 15 minutes, then again after 24 hours. The contents of the bottle are left to settle (usually for at least a week) and then the supernatant is decanted and filtered. The final pH should be 7.0 - 8.0.

Metadata

Last modified: 07.10.22
Source: CCAP
Medium type: Complex medium
Final pH: n.d.
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
KH2PO4 18.72 137.561
EDTA 0.04 0.137
KOH 0.025 0.442
ZnSO4 x 7 H2O 0.007 0.025
FeSO4 x 7 H2O 0.004 0.014
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