C17
17 .
BG11
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Mail sol. C17 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
NaNO3
(75 g/500 ml stock solution) |
10 | ml | - | - | |
|
K2HPO4
(2 g/500 ml stock solution) |
10 | ml | - | - | |
|
MgSO4 x 7 H2O
(3.75 g/500 ml stock solution) |
10 | ml | - | - | |
|
CaCl2 x 2 H2O
(1.8 g/500 ml stock solution) |
10 | ml | - | - | |
|
Citric acid
(0.3 g/500 ml stock solution) |
10 | ml | - | - | |
|
Ammonium ferric citrate
(green, 0.3 g/500 ml stock solution) |
10 | ml | - | - | |
|
Na2-EDTA
(0.05 g/500 ml stock solution) |
10 | ml | - | - | |
|
Na2CO3
(1 g/500 ml stock solution) |
10 | ml | 25.3 | 238.706 | |
| Trace metal solution | 1 | ml | - | - | |
| Agar , for solid medium | 15 | g | 15 | - | |
| 1 Make up to 1 litre with deionized water. Adjust pH to 7.1 with 1M NaOH or HCl. For agar add 15.0 g per litre of Bacteriological Agar (Thermo Scientific™ Oxoid™ Agar No.1). Autoclave at 15 psi for 15 minutes. | |||||
| 2 Due to precipitation, larger volumes require stocks of citric acid and ammonium ferric citrate to be autoclaved separately in 100ml deionized water or alternatively they can be autoclaved separately in test tubes and added to sterile medium in the airflow cabinet. | |||||
Solution occurs in 1
other media (first defined in Medium C17).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| H3BO3 | 2.86 | g | 2.86 | 46.248 |
| MnCl2 x 4 H2O | 1.81 | g | 1.81 | 9.146 |
| ZnSO4 x 7 H2O | 0.22 | g | 0.22 | 0.765 |
| Na2MoO4 x 2 H2O | 0.39 | g | 0.39 | 1.612 |
| CuSO4 x 5 H2O | 0.08 | g | 0.08 | 0.32 |
| Co(NO3)2 x 6 H2O | 0.05 | g | 0.05 | 0.172 |
Metadata
| Last modified: | 07.10.22 |
| Source: | CCAP |
| Synonyms: | Blue-Green Medium |
| Medium type: | Defined medium |
| Final pH: | 7.1 |
| Reference: | doi.org/10.1128/br.35.2.171-205.1971 |
| Edit in Medium builder | |