Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KNO3 | 0.10 | g | 0.1 | 0.989 | |
Ca(NO3)2 x 4 H2O | 0.15 | g | 0.15 | 0.635 | |
Sodium glycerophosphate | 0.05 | g | 0.05 | - | |
MgSO4 x 7 H2O | 0.04 | g | 0.04 | 0.162 | |
Tris(hydroxymethyl)aminomethane | 0.50 | g | 0.5 | 4.127 | |
Trace element solution | 3.00 | ml | - | - | |
Vitamin B1 solution | 1.00 | ml | - | - | |
Vitamin B12 solution | 1.00 | ml | - | - | |
Biotin solution | 10.00 | ml | - | - | |
Distilled water | 1000.00 | ml | - | - | |
Agar , for solid medium | 15.00 | g | 15 | - | |
1 Dissolve the TRIS buffer into 900 ml distilled water, then add the remaining components. Bring to 1 litre with distilled water. For agar add 15 g per litre Bacterial Agar. Autoclave at 15 psi for 15 minutes. Final pH should be 7.5. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Distilled water | 1000.00 | ml | - | - | |
Na2-EDTA | 0.75 | g | 0.75 | 2.231 | |
1 Add to 1000 ml of distilled water 0.75 g Na2EDTA and the minerals in exactly the following sequence: | |||||
FeCl3 x 6 H2O | 97.00 | mg | 0.097 | 0.359 | |
MnCl2 x 4 H2O | 41.00 | mg | 0.041 | 0.207 | |
ZnCl2 x 6 H20 | 5.00 | mg | 0.005 | 0.02 | |
CoCl2 x 6 H2O | 2.00 | mg | 0.002 | 0.015 | |
Na2MoO4 x 2 H2O | 4.00 | mg | 0.004 | 0.017 |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Thiamine hydrochloride
(Vitamin B1) |
0.12 | g | 1.2 | 3.558 | |
1 Filter sterile |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Cyanocobalamine
(Vitamin B12) |
0.1 | g | 1 | 0.738 | |
1 Take 1 ml of this solution and add 99 ml Deionised water. Filter sterile. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Biotin | 0.005 | g | 0.05 | 0.205 | |
Distilled water | 100.000 | ml | - | - | |
1 Filter sterile. |
Last modified: | 07.10.22 |
Source: | CCAP |
Medium type: | Defined medium |
Final pH: | 7.5 |
Description:
Reference: Ichimura T (1971) Sexual cell division and conjugationpapilla formation in sexual reproduction of Closterium strigosum. In: Nishizawa K, Arasaki S, Chihara M, Hirose H, Nakamura V, Tsuchiya Y eds. Proceedings of the Seventh International Seaweed Symposium, Sapporo, Japan, August 812, 1971. Proceedings of the Seventh International Seaweed Symposium. University of Tokyo Press, Tokyo, pp. 20814. - Watanabe MM, Kawachi M, Hiroki M & Kasai F (2000) NIES Collection List of Strains. Sixth Edition, 2000, Microalgae and Protozoa. Microbial Culture Collections, National Institute for Environmental Studies, Tsukuba, Japan, 159 pp. |
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