C34
34 .
f/2
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Medium for Chloromonas sp. CCAP6/2
* Modification: 20-25 °C; pH 7.5; lighting 50-400 microE
Mail sol. C34 #
ml
Solution occurs in 1
other media (first defined in Medium C34).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
NaNO3
(15 g/200 ml stock solution) |
1.0 | ml | - | - | |
|
NaH2PO4 x 2 H2O
(1.13 g/200 ml stock solution) |
1.0 | ml | - | - | |
| Trace elements (x10 concentration) | 0.1 | ml | - | - | |
| Vitamin mix | 1.0 | ml | - | - | |
|
Natural sea water
(filtered) |
1000.0 | ml | - | - | |
| Agar , for solid medium | 15.0 | g | 15 | - | |
| 1 Make up to 1 litre with filtered natural seawater. Adjust pH to 8.0 with 1M NaOH or 1 M HCl prior to autoclaving. For agar add 15g per litre Bacteriological Agar. Autoclave at 15 psi for 15 minutes. | |||||
Solution occurs in 4
other media (first defined in Medium C34).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Na2-EDTA | 8.320 | g | 41.6 | 123.734 |
| FeCl3 x 6 H2O | 6.300 | g | 31.5 | 116.539 |
| CuSO4 x 5 H2O | 0.020 | g | 0.1 | 0.401 |
| ZnSO4 x 7 H2O | 0.044 | g | 0.22 | 0.765 |
| CoCl2 x 6 H2O | 0.020 | g | 0.1 | 0.77 |
| MnCl2 x 4 H2O | 0.360 | g | 1.8 | 9.095 |
| Na2MoO4 x 2 H2O | 0.012 | g | 0.06 | 0.248 |
Solution occurs in 4
other media (first defined in Medium C34).
| 1 First make primary stocks of Cyanocobalamin and Biotin. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Cyanocobalamine
(0.01 g/100 ml stock solution) |
1.00 | ml | - | - | |
|
Biotin
(0.01 g/100 ml stock solution) |
1.00 | ml | - | - | |
| 2 Dispense any excess primary stocks into 1 ml aliquots and freeze. | |||||
| Thiamine HCl | 0.02 | g | 0.1 | 0.296 | |
Metadata
| Last modified: | 05.10.22 |
| Source: | CCAP |
| Medium type: | Complex medium |
| Final pH: | 8.0 |
| Reference: | doi.org/10.1139/m62-029 |
| Edit in Medium builder | |