Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
TES
(dry buffer) |
0.115 | g | 0.115 | 0.502 | |
CaCl2 x 2 H2O
(3.68 g/100 ml stock solution) |
1.000 | ml | - | - | |
MgSO4 x 7 H2O
(3.7 g/100 ml stock solution) |
1.000 | ml | - | - | |
NaHCO3
(1.26 g/100 ml stock solution) |
1.000 | ml | - | - | |
K2HPO4 x 3 H2O
(1.14 g/100 ml stock solution) |
1.000 | ml | - | - | |
NaNO3
(8.5 g/100 ml stock solution) |
1.000 | ml | - | - | |
Na2SiO3 x 5 H2O
(2.12 g/100 ml stock solution) |
1.000 | ml | - | - | |
Combined trace element solution* | 0.100 | ml | - | - | |
MWC Vitamin mix** | 1.000 | ml | - | - | |
Deionized water | 1000.000 | ml | - | - | |
1 Make up to 1 litre with deionized water. Autoclave at 15 psi for 15 minutes. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Na2-EDTA | 4.360 | g | 43.6 | 129.682 |
FeCl3 x 6 H2O | 3.150 | g | 31.5 | 116.539 |
CuSO4 x 5 H2O | 0.010 | g | 0.1 | 0.401 |
ZnSO4 x 7 H2O | 0.022 | g | 0.22 | 0.765 |
CoCl2 x 6 H2O | 0.010 | g | 0.1 | 0.77 |
MnCl2 x 4 H2O | 0.180 | g | 1.8 | 9.095 |
Na2MoO4 x 2 H2O | 0.006 | g | 0.06 | 0.248 |
H3BO3 | 1.000 | g | 10 | 161.708 |
1 First make primary stocks of Cyanocobalamin and Biotin. | |||||
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Cyanocobalamine
(0.01 g/100 ml stock solution) |
1.00 | ml | - | - | |
Biotin
(0.01 g/100 ml stock solution) |
1.00 | ml | - | - | |
2 Dispense any excess primary stocks into 1 ml aliquots and freeze. | |||||
Thiamine HCl | 0.02 | g | 0.1 | 0.296 |
Last modified: | 07.10.22 |
Source: | CCAP |
Synonyms: | Modified Woods Hole Medium |
Medium type: | Defined medium |
Final pH: | n.d. |
Reference: | doi.org/10.1111/j.1529-8817.1972.tb03995.x |
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