C98
98 .
SP
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Mail sol. C98 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Stock solution 1 | 500 | ml | - | - | |
| Stock solution 2 | 500 | ml | - | - | |
| 1 This medium is made up in 2 parts to reduce precipitation when autoclaving. For each part add the components to around 900 mls deionised water and then top up to 1 litre with deionised water. | |||||
| 2 Autoclave parts 1 and 2 separately at 15 psi, allow to cool then mix 1:1 aseptically. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaHCO3 | 27.22 | g | 27.22 | 324.021 |
| Na2CO3 | 8.06 | g | 8.06 | 76.046 |
| K2HPO4 | 1.00 | g | 1 | 5.741 |
| Deionized water | 1000.00 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaNO3 | 5.00 | g | 5 | 58.827 |
| K2SO4 | 2.00 | g | 2 | 11.477 |
| NaCl | 2.00 | g | 2 | 34.223 |
| MgSO4 x 7 H2O | 0.40 | g | 0.4 | 1.623 |
| CaCl2 x 2 H2O | 0.02 | g | 0.02 | 0.136 |
| FeSO4 x 7 H2O | 0.02 | g | 0.02 | 0.072 |
| Na2-EDTA | 0.16 | g | 0.16 | 0.476 |
| Micronutrient solution | 10.00 | ml | - | - |
| Vitamin solution | 5.00 | ml | - | - |
| Deionized water | 1000.00 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| ZnSO4 x 7 H2O | 1.00 | mg | 1.0e-3 | 0.003 | |
| MnSO4 x 7 H2O | 2.00 | mg | 0.002 | 0.013 | |
| H3BO3 | 10.00 | mg | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 1.00 | mg | 1.0e-3 | 0.004 | |
| Co(NO3)2 x 6 H2O | 1.00 | mg | 1.0e-3 | 0.003 | |
| CuSO4 x 5 H2O | 0.05 | mg | 5.0e-5 | 2.0e-4 | |
| FeSO4 x 7 H2O | 0.70 | g | 0.7 | 2.518 | |
| Na2-EDTA | 0.80 | g | 0.8 | 2.379 | |
| 1 May be stored frozen at -20 °C. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Biotin | 0.20 | mg | 2.0e-4 | 8.2e-4 | |
| Calcium pantothenate | 0.02 | g | 0.02 | 0.042 | |
| Cyanocobalamine | 4.00 | mg | 0.004 | 0.003 | |
| Folic acid | 0.40 | mg | 4.0e-4 | 9.1e-4 | |
| Inositol | 1.00 | g | 1 | 5.551 | |
| Nicotinic acid | 0.02 | g | 0.02 | 0.162 | |
| Thiamine HCl | 0.10 | g | 0.1 | 0.296 | |
| Thymine | 0.60 | g | 0.6 | 4.758 | |
| 1 May be stored frozen at -20 °C. | |||||
Metadata
| Last modified: | 07.10.22 |
| Source: | CCAP |
| Synonyms: | Spirulina Medium |
| Medium type: | Defined medium |
| Final pH: | n.d. |
| Edit in Medium builder | |