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JCM 1006 . HALOPHILIC SOB MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1006 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 1.00 g 0.962 7.284
MgSO4 x 7 H2O 1.00 g 0.962 3.905
CaCl2 x 2 H2O 0.30 g 0.289 1.964
KCl 0.60 g 0.577 7.746
NaCl 100.00 g 96.246 1646.93
Distilled water 1000.00 ml - -
1 Mix components thoroughly and autoclave. Aseptically add the following solutions (autoclaved or *filter-sterilized):
Na2S2O3
(1 M) 		20.00 ml - -
K2HPO4
(1 M) 		3.00 ml - -
NH4Cl
(1 M) 		5.00 ml - -
EDTA
(0.5 M) 		1.00 ml - -
NaHCO3
(*, 1 M) 		10.00 ml - -
Trace element solution
(see below, *) 		0.06 ml - -
Trace vitamins
(*) 		0.06 ml - -
Vitamin B12 solution 0.05 g/L
(*) 		6.00 �l - -
2 Adjust pH to 7.5.

Trace metal solution #

1001
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(32%) 		1.00 ml - -
FeCl2 x 4 H2O 2.00 g 1.998 10.05
H3BO3 0.05 g 0.05 0.808
ZnCl2 0.05 g 0.05 0.366
CuCl2 x 2 H2O 0.03 g 0.03 0.176
MnCl2 x 4 H2O 0.05 g 0.05 -
Na2MoO4 x 2 H2O 0.05 g 0.05 0.206
AlCl3 0.05 g 0.05 0.375
CoCl2 x 6 H2O 0.20 g 0.2 1.539
NiCl2 x 6 H2O 0.05 g 0.05 0.385
Na2SeO3 x 5 H2O 0.05 g 0.05 0.19
Na2WO4 x 2 H2O 0.05 g 0.05 0.151
Distilled water 1000.00 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Vitamin B12 solution 0.05 g/L #

100
Solution occurs in 18 other media (first defined in Medium J403).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 5 mg 0.05 0.037
Distilled water 100 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Medium type: Defined medium
Final pH: 7.5
Equipment needed: Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 96.246 1646.93
(NH4)2SO4 0.962 7.284
MgSO4 x 7 H2O 0.962 3.905
KCl 0.577 7.746
CaCl2 x 2 H2O 0.289 1.964
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