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J1009. CLOSTRIDIUM SWELLFUNIANUM MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.33 g 0.311 5.809
2 0.33 g 0.311 4.168
3 0.33 g 0.311 2.283
4
(BD-Difco)
0.50 g 0.471 -
5
(BD-Difco)
3.00 g 2.825 -
6 Trace element solution SL-10 1.00 ml - -
7 Trace vitamins 10.00 ml - -
8
(1%)
33.00 ml - -
9
(2%)
16.50 ml - -
10 0.50 g 0.471 2.681
11
(1%)
1.00 ml - -
12 1000.00 ml 939.931 52174.1
13
(w/v, 3%)
0.05 ml - -
14
(w/v, *, 10%)
0.05 ml 0.01 0.121
15
(w/v, 20%)
0.10 ml - -
Instructions:
  1. Adjust pH to 7.3.
  2. Prepare the medium anaerobically under a N2-CO2 (4:1, v/v) gas mixture. Distribute the medium into culture vessels (e.g., 5.0 ml in Hungate tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After autoclaving, add per 5.0 ml the following solutions from anaerobic stocks (autoclaved or *filter-sterilized and stored under a N2 atmosphere): #13-15
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 20 other media (first defined in medium J433 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(25%, 7.7 M)
10.0 ml 3.17 86.943
2 1.5 g 1.5 -
3 70.0 mg 0.07 0.514
4 100.0 mg 0.1 -
5 6.0 mg 0.006 0.097
6 190.0 mg 0.19 -
7 2.0 mg 0.002 -
8 24.0 mg 0.024 -
9 36.0 mg 0.036 0.149
10 990.0 ml 988.225 54854.8
Instructions:
  1. Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts.

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 950.261 52747.5
Tryptone 2.825 -
L-Cysteine x HCl x H2O 0.471 2.681
Yeast extract 0.471 -
NH4Cl 0.311 5.809
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