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JCM 1009 . CLOSTRIDIUM SWELLFUNIANUM MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1009 #

ml
Equipment needed: Anaerobic workbench Hungate tubes Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.33 g 0.311 5.809
KCl 0.33 g 0.311 4.168
KH2PO4 0.33 g 0.311 2.283
Yeast extract
(BD-Difco) 		0.50 g 0.471 -
Tryptone
(BD-Difco) 		3.00 g 2.825 39.742
Trace element solution SL-10 1.00 ml - -
Trace vitamins 10.00 ml - -
CaCl2 x 2 H2O
(1%) 		33.00 ml - -
MgCl2 x 6 H2O
(2%) 		16.50 ml - -
L-Cysteine HCl x H2O 0.50 g 0.471 -
Resazurin
(1%) 		1.00 ml - -
Distilled water 1000.00 ml - -
1 Prepare the medium anaerobically under a N2-CO2 (4:1, v/v) gas mixture. Distribute the medium into culture vessels (e.g., 5.0 ml in Hungate tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After autoclaving, add per 5.0 ml the following solutions from anaerobic stocks (autoclaved or *filter-sterilized and stored under a N2 atmosphere):
Na2S x 9 H2O
(w/v, 3%) 		0.05 ml - -
NaHCO3
(w/v, *, 10%) 		0.05 ml - -
Glucose
(w/v, 20%) 		0.10 ml - -
2 Adjust pH to 7.3.

Trace element solution SL-10 #

1000
Solution occurs in 20 other media (first defined in Medium J433).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%, 7.7 M) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.3
Equipment needed: Anaerobic workbench, Autoclave, Filter, Hungate tubes
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Tryptone 2.825 39.742
Yeast extract 0.471 -
L-Cysteine HCl x H2O 0.471 -
NH4Cl 0.311 5.809
KCl 0.311 4.168
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain