Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NH4Cl | 0.30 | g | 0.287 | 5.357 | |
K2HPO4 | 0.20 | g | 0.191 | 1.097 | |
KH2PO4 | 0.30 | g | 0.287 | 2.106 | |
KCl | 0.50 | g | 0.478 | 6.406 | |
NaCl | 1.00 | g | 0.955 | 16.343 | |
Yeast extract
(BD-Difco) |
2.00 | g | 1.91 | - | |
Trace element solution SL-10 | 1.00 | ml | - | - | |
Trace vitamins | 10.00 | ml | - | - | |
CaCl2 x 2 H2O
(1%) |
15.00 | ml | - | - | |
MgCl2 x 6 H2O
(2%) |
20.00 | ml | - | - | |
L-Cysteine HCl x H2O | 1.38 | g | 1.318 | - | |
Resazurin
(1%) |
1.00 | ml | - | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Prepare the medium anaerobically under a N2-CO2 (4:1, v/v) gas mixture. Distribute the medium into culture vessels (e.g., 5.0 ml in Hungate tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After autoclaving, add per 5.0 ml the following solutions from anaerobic stocks (autoclaved or *filter-sterilized and stored under a N2 atmosphere): | |||||
Na2S x 9 H2O
(w/v, 3%) |
0.10 | ml | - | - | |
NaHCO3
(w/v, *, 10%) |
0.25 | ml | - | - | |
L-Serine
(0.5 M) |
0.10 | ml | - | - | |
2 Adjust pH to 7.0 - 7.8. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
HCl
(25%, 7.7 M) |
10.0 | ml | 2.5 | 68.568 | |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
Distilled water | 990.0 | ml | - | - | |
1 Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Medium type: | Complex medium |
Final pH: | 7.4 |
Equipment needed: | Anaerobic workbench, Autoclave, Filter, Hungate tubes |
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