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JCM 1014 . NASU IRON REDUCER MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1014 #

ml
Equipment needed: Filter Autoclave Anaerobic workbench
1 Mix components thoroughly and adjust pH to 6.0. Autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions anaerobically and aseptically (autoclaved or *filter-sterilized and store under a N2 atmosphere):
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 0.20 g 0.194 1.468
KH2PO4 3.00 g 2.91 21.382
MgSO4 x 7 H2O 0.50 g 0.485 1.968
CaCl2 x 2 H2O 0.25 g 0.242 1.649
Yeast extract 0.50 g 0.485 -
Modified Allen's trace metal solution 1.00 ml - -
Trace vitamins
(*) 		10.00 ml - -
Iron(III) citrate solution 100.00 ml - -
Distilled water 900.00 ml - -
L-Cysteine HCl
(25 mM) 		10.00 ml - -
FeCl2
(130 mM) 		10.00 ml - -

Modified Allen's trace metal solution #

1000
Solution occurs in 1 other media (first defined in Medium J1014).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MnCl2 x 4 H2O 1.80 g 1.8 -
Na2B4O7 x 10 H2O 4.50 g 4.5 -
ZnSO4 x 7 H2O 0.22 g 0.22 0.765
CuCl2 x 2 H2O 0.05 g 0.05 0.293
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
VOSO4 x n H2O 0.03 g 0.03 -
CoSO4 x 7 H2O 0.01 g 0.01 0.065
Distilled water 1000.00 ml - -

Iron(III) citrate solution #

Solution occurs in 2 other media (first defined in Medium J1014).
1 Mix 2.4 g iron(III) citrate and 90 ml distilled water, and boil until dissolved. Cool to an ambient temperature and adjust pH to 6.0 with 10 N NaOH solution. Fill up to 100 ml with distilled water.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.0
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
KH2PO4 2.91 21.382
MgSO4 x 7 H2O 0.485 1.968
Yeast extract 0.485 -
CaCl2 x 2 H2O 0.242 1.649
(NH4)2SO4 0.194 1.468
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Cultivation metadata from 1 strains BacDive

halophily non-halophilic 1 strain
pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
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