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JCM 1017 . PFENNIG THERMOFILUM MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1017 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.33 g 0.321 6.007
KH2PO4 0.33 g 0.321 2.361
KCl 0.33 g 0.321 4.31
CaCl2 x 2 H2O 0.33 g 0.321 2.186
MgCl2 x 6 H2O 0.33 g 0.321 1.581
Trace element solution 1.00 ml - -
Trace vitamins 10.00 ml - -
Glucose 2.00 g 1.947 10.809
Yeast extract
(Oxoid) 		0.10 g 0.097 -
Trypticase peptone
(BD-BBL) 		0.50 g 0.487 -
Archaeal culture filtrate 10.00 ml - -
Resazurin 1.00 mg 9.7e-4 0.004
Distilled water 1000.00 ml - -
1 Mix components thoroughly, adjust pH to 6.0 and autoclave under a N2 gas atmosphere. Before inoculation, add per liter the following solution (autoclaved and stored under a N2 gas atmosphere):
Na2S x 9 H2O
(neutralized, 5%) 		6.00 ml - -

Archaeal culture filtrate #

Equipment needed: Filter
1 Cultivate Desulfurococcus kamchatkensis strain 1221n (=JCM 16383) in the above described medium supplemented with 0.6 g/L (final) yeast extract (without the archaeal culture filtrate). After three days of the cultivation, filtrate the grown culture with 0.22 �m (pore size) filter.
2 Comment: For cultivation of JCM 19810, increase amount of yeast extract to 1.0 g/L. Growth may be affected by quality of yeast extract.

Trace element solution #

1000
Solution occurs in 2 other media (first defined in Medium J899).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Fe(NH4)2(SO4)2 x 6 H2O 784 mg 0.784 -
CoCl2 x 6 H2O 238 mg 0.238 1.833
(NH4)2Ni(SO4)2 x 6 H2O 395 mg 0.395 1
Na2MoO4 x 2 H2O 24 mg 0.024 0.099
Na2WO4 x 2 H2O 33 mg 0.033 0.1
ZnSO4 x 7 H2O 144 mg 0.144 0.501
CuCl2 x 2 H2O 2 mg 0.002 0.012
Na2SeO4 94 mg 0.094 0.497
H3BO3 6 mg 0.006 0.097
MnCl2 x 4 H2O 99 mg 0.099 -
Distilled water 1000 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 6.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glucose 1.947 10.809
Trypticase peptone 0.487 -
NH4Cl 0.321 6.007
KCl 0.321 4.31
KH2PO4 0.321 2.361
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature hyperthermophilic 1 strain