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JCM 1019 . MMJHS-2 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1019 #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Modified MJ synthetic seawater B 1000 ml - -
NaNO3 1 g 1 11.765
Na2S2O3 x 5 H2O 1 g 1 4.029
NaHCO3 1 g 1 11.904
Sulfur
(powder) 		10 g 10 311.818
1 Mix components, except sulfur and NaHCO3, and autoclave. Steam sulfur for 3 hr on each of 3 successive days. Filter-sterilize 8% (w/v) NaHCO3 solution and add to the medium. Aseptically distribute the medium into culture vessels containing appropriate amount of sulfur (e.g., 20 ml of the medium with 0.2 g sulfur in 100 ml serum bottle) and seal with butyl rubber stoppers. Replace gas phase with a H2-CO2 (4:1, v/v) gas mixture and check medium pH to be 6.0. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).

Modified MJ synthetic seawater B #

1010
Solution occurs in 1 other media (first defined in Medium J892).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 25.00 g 24.753 423.554
MgCl2 x 6 H2O 4.20 g 4.158 20.454
MgSO4 x 7 H2O 3.40 g 3.366 13.658
KCl 0.50 g 0.495 6.641
NH4Cl 0.25 g 0.248 4.628
K2HPO4 0.14 g 0.139 0.796
CaCl2 x 2 H2O 0.70 g 0.693 4.714
NiCl2 x 6 H2O 1.00 mg 9.9e-4 0.008
Na2SeO3 x 5 H2O 1.00 mg 9.9e-4 0.004
Trace mineral solution 10.00 ml - -
Distilled water 1000.00 ml - -

Trace mineral solution #

1000
Solution occurs in 1 other media (first defined in Medium J892).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace minerals 1000.000 ml - -
NiCl2 x 6 H2O 0.025 g 0.025 0.193
Na2SeO3 x 5 H2O 0.300 mg 3.0e-4 0.001

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 24.762 423.723
Sulfur 10 311.818
MgCl2 x 6 H2O 4.158 20.454
MgSO4 x 7 H2O 3.396 13.778
NaHCO3 1 11.904
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Cultivation metadata from 3 strains BacDive

phyla 2 3 strains
oxygen microaerophile 1 strain
temperature thermophilic 2 strains
temperature mesophilic 1 strain