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J1020. DESULFOCARBO INDIANENSIS MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.000 g 1.843 12.977
2 8.800 g 8.111 138.779
3 4.600 g 4.24 20.854
4 0.340 g 0.313 4.203
5 0.250 g 0.23 4.308
6 0.140 g 0.129 0.948
7 0.140 g 0.129 0.878
8 9.300 mg 0.009 -
9 2.700 mg 0.002 -
10 0.260 mg 2.4e-4 -
11 0.084 mg 7.7e-5 -
12 Trace element solution 10.000 ml - -
13 Trace vitamins 10.000 ml - -
14 0.360 g 0.332 -
15 1.000 mg 9.2e-4 0.004
16 1000.000 ml 920.006 51068.1
17
(filter-sterilized, 8%)
60.000 ml 9.551 113.697
18
(autoclaved, 5%)
5.000 ml - -
Instructions:
  1. Mix components thoroughly and distribute the medium into culture vessels (e.g., 10 ml medium in Balch-type tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions: #17-18
Solution occurs in 89 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 70 mg 0.07 0.514
2 100 mg 0.1 -
3 6 mg 0.006 0.097
4 190 mg 0.19 -
5 2 mg 0.002 -
6 24 mg 0.024 -
7 36 mg 0.036 0.149
8 1000 ml 998.207 55408.8
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 938.406 52089.5
NaHCO3 9.551 113.697
NaCl 8.111 138.779
MgCl2 x 6H2O 4.24 20.854
Na2SO4 1.843 12.977
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